Detailed information of TA system TA035162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 181217..181953
• Replicon: plasmid pWP8-W19-CRE-01_1
• Accession: NZ_AP022269
• Organism: Raoultella ornithinolytica strain WP8-W19-CRE-01

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: H7R88_RS26815
• Protein ID: WP_183114670.1
• Coordinates: 181471..181953 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: H7R88_RS26810
• Protein ID: WP_003026799.1
• Coordinates: 181217..181483 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R88_RS26765	176397..177398	+	1002	Protein_202	Abi family protein	-
H7R88_RS26770	177537..178115	-	579	WP_009654302.1	hypothetical protein	-
H7R88_RS26775	178405..178668	+	264	WP_009310051.1	hypothetical protein	-
H7R88_RS26780	178665..179231	+	567	WP_009654312.1	hypothetical protein	-
H7R88_RS26785	179262..179756	+	495	WP_013023776.1	hypothetical protein	-
H7R88_RS26790	179800..180168	+	369	WP_183114669.1	hypothetical protein	-
H7R88_RS26795	180202..180405	+	204	WP_004098931.1	hemolysin expression modulator Hha	-
H7R88_RS26800	180454..180711	+	258	WP_009653918.1	hypothetical protein	-
H7R88_RS26805	180787..181041	+	255	WP_009653916.1	hypothetical protein	-
H7R88_RS26810	181217..181483	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
H7R88_RS26815	181471..181953	+	483	WP_183114670.1	GNAT family N-acetyltransferase	Toxin
H7R88_RS26820	182153..183556	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
H7R88_RS26825	183585..184217	-	633	WP_001567369.1	hypothetical protein	-
H7R88_RS26830	184443..185789	+	1347	WP_077267029.1	ISNCY family transposase	-
H7R88_RS26835	185838..186242	+	405	WP_064145647.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-5	-	1..241445	241445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17313.02 Da
• Isoelectric Point: 9.5893

>T35162 WP_183114670.1 NZ_AP022269:181471-181953 [Raoultella ornithinolytica]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHGKGIGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHKRTLFLKLQ

Nucleotide

• Length: 483 bp

>T35162 NZ_AP022269:181471-181953 [Raoultella ornithinolytica]
GTGGGATGTATAACAGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCCGAGTTCGTCAGTGGAGAAACGGTACTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAGGCTCTTGGTGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTTGCTCGCCTGGCGGTAGATATCTCATTGCACGGAAAAGGGATTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGCTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTCATAAGCGGACTCTGTTCCTTAAACTTCAA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT35162 WP_003026799.1 NZ_AP022269:181217-181483 [Raoultella ornithinolytica]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT35162 NZ_AP022269:181217-181483 [Raoultella ornithinolytica]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCGCCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66