Detailed information of TA system TA035159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4795857..4796421
• Replicon: chromosome
• Accession: NZ_AP022268
• Organism: Raoultella ornithinolytica strain WP8-W19-CRE-01

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: H7R88_RS22580
• Protein ID: WP_183114417.1
• Coordinates: 4796146..4796421 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: H7R88_RS22575
• Protein ID: WP_113991318.1
• Coordinates: 4795857..4796159 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R88_RS22560	4791799..4792134	+	336	WP_183114416.1	endoribonuclease SymE	-
H7R88_RS22565	4792463..4794439	+	1977	WP_183114659.1	AAA family ATPase	-
H7R88_RS22570	4794439..4795761	+	1323	WP_162661703.1	restriction endonuclease	-
H7R88_RS22575	4795857..4796159	-	303	WP_113991318.1	BrnA antitoxin family protein	Antitoxin
H7R88_RS22580	4796146..4796421	-	276	WP_183114417.1	BrnT family toxin	Toxin
H7R88_RS22585	4796538..4796957	-	420	WP_004857515.1	FosA family fosfomycin resistance glutathione transferase	-
H7R88_RS22590	4796951..4797859	-	909	WP_004857513.1	LysR family transcriptional regulator	-
H7R88_RS22595	4797947..4798729	+	783	WP_104896592.1	NAD(P)H-dependent oxidoreductase	-
H7R88_RS22600	4798877..4799455	+	579	WP_113991322.1	TetR/AcrR family transcriptional regulator	-
H7R88_RS22605	4799599..4800411	+	813	WP_183114418.1	winged helix-turn-helix domain-containing protein	-
H7R88_RS22610	4800408..4800926	+	519	WP_015585229.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-87)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (35-93)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10587.95 Da
• Isoelectric Point: 7.0737

>T35159 WP_183114417.1 NZ_AP022268:c4796421-4796146 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFNDPQHLSRHDRYENGEYRWQTIGLVHGVIVVLVAHSIRFESGTEVIRIIS
ARKADKNYEHG

Nucleotide

• Length: 276 bp

>T35159 NZ_AP022268:c4796421-4796146 [Raoultella ornithinolytica]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAGCAAGCAATCTAAGAAAACATGGGATTCGCTTCGAGGAGGCGGT
ACTGGTTTTTAACGATCCGCAGCATCTTTCCCGACATGATCGCTATGAAAATGGTGAGTATCGATGGCAAACCATCGGGC
TTGTTCATGGCGTGATTGTTGTTCTTGTGGCGCATTCGATCCGTTTTGAAAGTGGAACGGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAAAAATTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11539.05 Da
• Isoelectric Point: 7.0165

>AT35159 WP_113991318.1 NZ_AP022268:c4796159-4795857 [Raoultella ornithinolytica]
MSMVKHKRDNPTALTAQREDELKALAKKSDDDIDYSDIPSSDDEPWSEAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIRDLSEK

Nucleotide

• Length: 303 bp

>AT35159 NZ_AP022268:c4796159-4795857 [Raoultella ornithinolytica]
ATGAGCATGGTTAAACATAAACGCGATAACCCGACAGCGCTTACCGCTCAGCGTGAAGACGAACTCAAAGCGCTGGCGAA
AAAATCAGATGATGACATTGATTACAGCGATATCCCCTCTTCGGACGATGAGCCGTGGTCAGAGGCGGTTCGTGGTAAAT
TTTATCGGCCATTAAAAACGCAGGCCTCTGTGCGCATCGATGCAGATGTGATGGAGTGGCTTAAACGCCCGGGAAAAGGC
TATCAAACGCGGCTGAACGCGATTTTAAGAGAAGCGATGATTCGCGATCTGAGCGAGAAATAA