Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4219773..4220392 Replicon chromosome
Accession NZ_AP022268
Organism Raoultella ornithinolytica strain WP8-W19-CRE-01

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag H7R88_RS19885 Protein ID WP_004858783.1
Coordinates 4220174..4220392 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag H7R88_RS19880 Protein ID WP_004858785.1
Coordinates 4219773..4220147 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R88_RS19870 4214883..4216076 + 1194 WP_004858789.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H7R88_RS19875 4216099..4219245 + 3147 WP_004858787.1 multidrug efflux RND transporter permease subunit -
H7R88_RS19880 4219773..4220147 + 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
H7R88_RS19885 4220174..4220392 + 219 WP_004858783.1 hemolysin expression modulator Hha Toxin
H7R88_RS19890 4220544..4221110 + 567 WP_004858781.1 maltose O-acetyltransferase -
H7R88_RS19895 4221210..4221680 + 471 WP_004858780.1 YlaC family protein -
H7R88_RS19900 4221655..4223106 - 1452 WP_071359190.1 PLP-dependent aminotransferase family protein -
H7R88_RS19905 4223211..4223921 + 711 WP_015585015.1 GNAT family N-acetyltransferase -
H7R88_RS19910 4223918..4224058 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
H7R88_RS19915 4224061..4224321 - 261 WP_004858777.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T35158 WP_004858783.1 NZ_AP022268:4220174-4220392 [Raoultella ornithinolytica]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T35158 NZ_AP022268:4220174-4220392 [Raoultella ornithinolytica]
ATGTCTGGTGAACCATTAACCAAAACAGACTATTTGATGCGCTTACGTCGCTGTCAGACAATTGATACGCTGGAACGCGT
AATTGAAAAAAACAAATATGAACTTTCAGATAACGAACTGGCTGTATTTTACTCAGCGGCCGACCACCGTCTTGCTGAAT
TGACCATGAATAAGCTCTACGACAAAATCCCGACATCGGTGTGGAAGTTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT35158 WP_004858785.1 NZ_AP022268:4219773-4220147 [Raoultella ornithinolytica]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT35158 NZ_AP022268:4219773-4220147 [Raoultella ornithinolytica]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCGCAGCTTCGATTTCTTTGCGAAACCCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGTAACCACGGGTGGGTGAATGATCCAACGTCGGCTATCAATCTTCAACTTAATGAATTGATCGAAC
ACATAGCCACGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAACTGATCGCTCAGCTGGACGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGCATTAACACGGCCGATCTACAGAAATGGCGCAAATCGGGAAATCGGTT
ATTCCGATGTTTTGTCAACGCCAGTCGGGAAAATCCGGCAAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References