Detailed information of TA system TA035157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2725508..2727111
• Replicon: chromosome
• Accession: NZ_AP022268
• Organism: Raoultella ornithinolytica strain WP8-W19-CRE-01

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: H7R88_RS12780
• Protein ID: WP_183114639.1
• Coordinates: 2725508..2726839 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A808YAX5
• Locus tag: H7R88_RS12785
• Protein ID: WP_032717448.1
• Coordinates: 2726839..2727111 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R88_RS12765	2721382..2722296	-	915	WP_015584333.1	branched-chain amino acid ABC transporter permease	-
H7R88_RS12770	2722396..2723454	-	1059	WP_162180097.1	branched-chain amino acid ABC transporter substrate-binding protein	-
H7R88_RS12775	2724169..2725575	+	1407	WP_183114649.1	glycoside hydrolase family 10 protein	-
H7R88_RS12780	2725508..2726839	-	1332	WP_183114639.1	type II toxin-antitoxin system HipA family toxin	Toxin
H7R88_RS12785	2726839..2727111	-	273	WP_032717448.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
H7R88_RS12790	2727314..2727868	-	555	WP_004861926.1	RNA polymerase sigma factor	-
H7R88_RS12795	2727874..2728299	-	426	WP_032724724.1	hypothetical protein	-
H7R88_RS12800	2728432..2729385	-	954	WP_004861921.1	hypothetical protein	-
H7R88_RS12805	2729599..2730231	-	633	WP_069475943.1	class I SAM-dependent methyltransferase	-
H7R88_RS12810	2730251..2731492	-	1242	WP_004861912.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2683236..2859883	176647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49357.68 Da
• Isoelectric Point: 6.7012

>T35157 WP_183114639.1 NZ_AP022268:c2726839-2725508 [Raoultella ornithinolytica]
MATLVTWMNNERVGELIKQANGAHLFRYEEAWMRNPRARPLSLSLPLQYGNITDEAVFNYFDNLLPDSPRVRDRIVKRYQ
ARSKQPFDLLAEIGRDSVGAVTLLPPGENAPRGALSWEPLDNHALDALLTAYQSDIPLGMVNEHDDFRISVAGAQEKTAL
LKIGEQWCIPTGTTPTTHIIKLPIGEIKQPDAVLDLSESVDNEYLCLALARALGFAVPPASIIQTASIRALAVERFDRRW
AQEKTVLLRLPQEDLCQAFGIPSSIKYESDGGPGIRAIMAFLVGSSEALEDRYNFMKFMVFQWLIGATDGHAKNFSIFLQ
PGGSYRLTPFYDIISAFPLLGGKGLHLSDLKLSMSLKASKGRKTEIQTLYPRHFLATAKEVGFAQQQMLEILRYFVDNVP
RAVEAVKGTLPPDFSQPVYEAITDRLLWVHSRLQNAMAASPSS

Nucleotide

• Length: 1332 bp

>T35157 NZ_AP022268:c2726839-2725508 [Raoultella ornithinolytica]
ATGGCGACTCTCGTGACATGGATGAACAATGAGCGGGTCGGCGAGCTAATCAAGCAGGCGAACGGCGCACATCTCTTTCG
CTATGAAGAGGCGTGGATGCGCAATCCGCGCGCCAGACCGCTCTCACTGTCGCTTCCGCTGCAGTACGGTAACATCACCG
ACGAGGCGGTATTTAACTACTTTGACAATCTGCTGCCGGACAGTCCGCGGGTACGGGACCGCATTGTTAAGCGCTATCAG
GCGCGTTCAAAGCAGCCGTTTGATCTGCTGGCCGAGATCGGACGGGACAGCGTCGGGGCGGTAACGTTACTCCCGCCGGG
AGAAAATGCGCCCCGCGGTGCGTTGAGCTGGGAGCCGCTGGATAACCACGCTCTGGATGCGTTACTGACGGCCTACCAGT
CGGATATACCGCTCGGAATGGTCAACGAACACGATGATTTCAGGATCTCGGTGGCCGGGGCCCAGGAGAAAACCGCGCTG
CTGAAAATCGGCGAGCAGTGGTGTATCCCGACGGGTACCACGCCGACCACCCACATCATTAAACTGCCTATCGGCGAGAT
CAAACAGCCGGATGCGGTGCTGGATCTCAGCGAAAGCGTCGATAACGAATATTTGTGTCTGGCGCTGGCCCGGGCGCTGG
GTTTTGCCGTTCCGCCCGCCAGCATTATCCAGACAGCCAGTATCAGGGCGCTGGCGGTGGAGCGTTTCGACCGTCGCTGG
GCGCAGGAGAAAACGGTGCTGCTGCGTTTGCCGCAGGAAGATCTGTGTCAGGCCTTCGGCATTCCGTCATCGATTAAGTA
TGAGTCCGACGGCGGGCCGGGCATTCGGGCAATTATGGCTTTTCTGGTTGGCTCCAGCGAGGCACTCGAGGATCGCTATA
ACTTTATGAAGTTTATGGTGTTCCAGTGGCTGATTGGCGCAACCGATGGCCACGCCAAAAACTTCTCGATATTCCTCCAG
CCGGGCGGCAGCTACCGCTTAACCCCGTTCTACGACATTATCTCCGCGTTTCCGCTGCTTGGCGGAAAGGGACTGCACCT
GAGCGATCTCAAGCTGTCGATGAGTCTGAAGGCCAGTAAAGGACGTAAAACGGAGATCCAGACGCTCTATCCGCGTCACT
TTCTCGCTACGGCTAAAGAGGTAGGGTTTGCCCAACAGCAGATGCTGGAGATTCTGCGCTATTTTGTCGATAACGTTCCC
CGCGCGGTTGAGGCGGTAAAGGGGACTTTACCGCCTGACTTTTCACAACCGGTATATGAGGCGATCACCGATCGCTTACT
GTGGGTCCATTCACGCTTGCAGAACGCAATGGCGGCGTCTCCGTCGAGCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10365.71 Da
• Isoelectric Point: 5.7971

>AT35157 WP_032717448.1 NZ_AP022268:c2727111-2726839 [Raoultella ornithinolytica]
MSPSPTIYSPVQLANYLKLVRQKNQWTQSELAQRMGIKQATVSNFENHPEKTTLTTVFKILQSLELSMAICEKNAPDTDD
NRDNPRDLEW

Nucleotide

• Length: 273 bp

>AT35157 NZ_AP022268:c2727111-2726839 [Raoultella ornithinolytica]
ATGAGCCCTTCACCAACGATATACAGCCCGGTTCAGCTGGCAAACTATCTCAAGCTGGTGCGCCAGAAAAACCAGTGGAC
GCAGAGCGAGCTGGCTCAGCGTATGGGGATTAAACAGGCCACCGTCTCTAACTTTGAAAATCATCCCGAGAAAACGACGT
TAACCACGGTGTTTAAAATTTTACAGTCACTGGAACTGTCGATGGCGATTTGCGAAAAAAATGCGCCGGATACCGACGAT
AACCGTGATAACCCGCGGGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A808YAX5