Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 74794..75341 Replicon chromosome
Accession NZ_AP022268
Organism Raoultella ornithinolytica strain WP8-W19-CRE-01

Toxin (Protein)


Gene name ccdB Uniprot ID A0A808XUV0
Locus tag H7R88_RS00350 Protein ID WP_048025274.1
Coordinates 74794..75102 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A808XYT1
Locus tag H7R88_RS00355 Protein ID WP_004869059.1
Coordinates 75105..75341 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R88_RS00320 69982..70722 + 741 WP_015585491.1 4-oxalmesaconate hydratase -
H7R88_RS00325 70719..71432 + 714 WP_015585492.1 4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase -
H7R88_RS00330 71437..72528 + 1092 WP_015585493.1 4-oxalomesaconate tautomerase -
H7R88_RS00335 72525..73391 + 867 WP_015585494.1 NAD(P)-dependent oxidoreductase -
H7R88_RS00340 73392..73667 - 276 WP_162769256.1 hypothetical protein -
H7R88_RS00345 73776..74678 - 903 WP_015585496.1 EamA family transporter -
H7R88_RS00350 74794..75102 - 309 WP_048025274.1 CcdB family protein Toxin
H7R88_RS00355 75105..75341 - 237 WP_004869059.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H7R88_RS00360 75447..76880 - 1434 WP_004869058.1 PTS N-acetylmuramic acid transporter subunit IIBC -
H7R88_RS00365 76905..77807 - 903 WP_088402359.1 N-acetylmuramic acid 6-phosphate etherase -
H7R88_RS00370 77972..79135 - 1164 WP_044350982.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11778.62 Da        Isoelectric Point: 6.7156

>T35143 WP_048025274.1 NZ_AP022268:c75102-74794 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRVVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Download         Length: 309 bp

>T35143 NZ_AP022268:c75102-74794 [Raoultella ornithinolytica]
ATACAGTATTACGTTTATAAAAATACCGGTCGAATTACTGTGTATCCTTATCTCCTTGATGTACAAAGTGACATTATTGG
AATGCGCAATACCCGGGTCGTGATCCCACTATTCCCGCTGAGAAATTACAAAGGTCCGCGCGCCGATCGTTTAACACCAG
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACAACGCGTTCTGGGGGAAGAG
GTTTGTCATCTCAATCACCAACGTGAAGTCGTTAAAGCATCAATAGATTTTCTTTTTGACGGGATTTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8898.08 Da        Isoelectric Point: 5.6579

>AT35143 WP_004869059.1 NZ_AP022268:c75341-75105 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT35143 NZ_AP022268:c75341-75105 [Raoultella ornithinolytica]
ATGCCAGCCGTAAAACACAATAAGCAGAATGTCAGCGTGACGCTGGATCCTATACTGGTCAAACAGGCTCGTGAGATCGG
CATCAATCTTTCTCAAACGTTGACGAGCGCTCTAAAAAGCGAAATCCACAGCATCGAAATGGCACGCTGGCAAAAAGAAA
ATGCCAGGGCAATCGACTACCTCAATGATTTAACAGAGGAAAATGGACTTCTCTCTGACGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808XUV0

Antitoxin

Source ID Structure
AlphaFold DB A0A808XYT1

References