Detailed information of TA system TA035133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4251397..4251992
• Replicon: chromosome
• Accession: NZ_AP022261
• Organism: Escherichia coli strain WP8-S18-ESBL-07

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: H7R25_RS20155
• Protein ID: WP_000239581.1
• Coordinates: 4251397..4251747 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: H7R25_RS20160
• Protein ID: WP_001223213.1
• Coordinates: 4251741..4251992 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R25_RS20135	4246851..4247873	-	1023	WP_157940635.1	ABC transporter permease	-
H7R25_RS20140	4247887..4249389	-	1503	WP_089575097.1	sugar ABC transporter ATP-binding protein	-
H7R25_RS20145	4249522..4250478	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
H7R25_RS20150	4250788..4251318	+	531	WP_000055075.1	inorganic diphosphatase	-
H7R25_RS20155	4251397..4251747	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
H7R25_RS20160	4251741..4251992	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
H7R25_RS20165	4252204..4252545	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
H7R25_RS20170	4252548..4256327	-	3780	WP_160420465.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T35133 WP_000239581.1 NZ_AP022261:c4251747-4251397 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T35133 NZ_AP022261:c4251747-4251397 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT35133 WP_001223213.1 NZ_AP022261:c4251992-4251741 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT35133 NZ_AP022261:c4251992-4251741 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGACGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAATGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7