Detailed information of TA system TA035128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3128940..3129645
• Replicon: chromosome
• Accession: NZ_AP022261
• Organism: Escherichia coli strain WP8-S18-ESBL-07

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: H7R25_RS14985
• Protein ID: WP_000539521.1
• Coordinates: 3128940..3129326 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H7R25_RS14990
• Protein ID: WP_001280945.1
• Coordinates: 3129316..3129645 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R25_RS14965	3124944..3125570	+	627	WP_001314584.1	glutathione S-transferase GstB	-
H7R25_RS14970	3125567..3126682	-	1116	WP_000555033.1	aldose sugar dehydrogenase YliI	-
H7R25_RS14975	3126793..3127176	-	384	WP_000497137.1	biofilm formation regulator BssR	-
H7R25_RS14980	3127389..3128714	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
H7R25_RS14985	3128940..3129326	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R25_RS14990	3129316..3129645	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
H7R25_RS14995	3129715..3131043	-	1329	WP_001544398.1	GGDEF domain-containing protein	-
H7R25_RS15000	3131051..3133399	-	2349	WP_001322378.1	EAL domain-containing protein	-
H7R25_RS15005	3133577..3134488	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T35128 WP_000539521.1 NZ_AP022261:3128940-3129326 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T35128 NZ_AP022261:3128940-3129326 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT35128 WP_001280945.1 NZ_AP022261:3129316-3129645 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT35128 NZ_AP022261:3129316-3129645 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGACCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6