Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2531267..2531905 Replicon chromosome
Accession NZ_AP022261
Organism Escherichia coli strain WP8-S18-ESBL-07

Toxin (Protein)


Gene name hicA Uniprot ID B7N4J4
Locus tag H7R25_RS12055 Protein ID WP_000813797.1
Coordinates 2531729..2531905 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H7R25_RS12050 Protein ID WP_076611057.1
Coordinates 2531267..2531683 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R25_RS12030 2526419..2527360 - 942 WP_001251335.1 ABC transporter permease -
H7R25_RS12035 2527361..2528374 - 1014 WP_000220413.1 ABC transporter ATP-binding protein -
H7R25_RS12040 2528392..2529537 - 1146 WP_000047447.1 ABC transporter substrate-binding protein -
H7R25_RS12045 2529782..2531188 - 1407 WP_001544550.1 PLP-dependent aminotransferase family protein -
H7R25_RS12050 2531267..2531683 - 417 WP_076611057.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H7R25_RS12055 2531729..2531905 - 177 WP_000813797.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H7R25_RS12060 2532127..2532357 + 231 WP_000494244.1 YncJ family protein -
H7R25_RS12065 2532449..2534410 - 1962 WP_001309488.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H7R25_RS12070 2534483..2535019 - 537 WP_000429161.1 DNA-binding transcriptional regulator SutR -
H7R25_RS12075 2535072..2536283 + 1212 WP_001323162.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6724.76 Da        Isoelectric Point: 11.5666

>T35126 WP_000813797.1 NZ_AP022261:c2531905-2531729 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T35126 NZ_AP022261:c2531905-2531729 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15251.65 Da        Isoelectric Point: 4.5908

>AT35126 WP_076611057.1 NZ_AP022261:c2531683-2531267 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT35126 NZ_AP022261:c2531683-2531267 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J715

Antitoxin

Source ID Structure

References