Detailed information of TA system TA035110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 67469..68106
• Replicon: plasmid pWP8-S18-ESBL-06_2
• Accession: NZ_AP022258
• Organism: Klebsiella sp. WP8-S18-ESBL-06

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: H7R76_RS24710
• Protein ID: WP_031624380.1
• Coordinates: 67696..68106 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: H7R76_RS24705
• Protein ID: WP_182968128.1
• Coordinates: 67469..67699 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R76_RS24675	62746..63612	-	867	WP_004118283.1	replication initiation protein	-
H7R76_RS24680	64147..64251	+	105	WP_032409716.1	hypothetical protein	-
H7R76_RS24685	64380..64637	+	258	WP_000764642.1	hypothetical protein	-
H7R76_RS24690	64695..65471	-	777	WP_182968138.1	site-specific integrase	-
H7R76_RS24695	66314..66619	-	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	-
H7R76_RS24700	66621..66839	-	219	WP_023294775.1	type II toxin-antitoxin system antitoxin CcdA	-
H7R76_RS24705	67469..67699	+	231	WP_182968128.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H7R76_RS24710	67696..68106	+	411	WP_031624380.1	PIN domain-containing protein	Toxin
H7R76_RS24715	68433..69413	-	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
H7R76_RS24720	69484..70290	-	807	Protein_75	IS5 family transposase	-
H7R76_RS24725	70568..70849	-	282	WP_000780222.1	helix-turn-helix transcriptional regulator	-
H7R76_RS24730	70830..71159	-	330	WP_000493286.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..72104	72104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-118)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14321.49 Da
• Isoelectric Point: 4.4978

>T35110 WP_031624380.1 NZ_AP022258:67696-68106 [Klebsiella sp. WP8-S18-ESBL-06]
VNRIYMLDTGICTFILREQPEAVIKRLEQAVLAGDRVVISAITWAGISLAARVSGPAAEQLADAFAARLDAILPWDRAAV
DATTEIKVALRLAGTSIGPNDTAIAGHAIAAGAVLVTNSVGEFERVPGLVMEDWVN

Nucleotide

• Length: 411 bp

>T35110 NZ_AP022258:67696-68106 [Klebsiella sp. WP8-S18-ESBL-06]
GTGAACAGAATCTATATGCTCGACACCGGTATCTGCACGTTCATCCTGCGCGAGCAGCCGGAAGCCGTCATTAAACGCCT
GGAGCAGGCAGTGCTGGCCGGCGATCGGGTTGTGATCTCCGCCATCACCTGGGCCGGGATTTCTCTGGCCGCCCGGGTGT
CAGGCCCTGCCGCTGAGCAGCTGGCGGATGCCTTCGCTGCCCGCCTTGATGCCATCCTGCCCTGGGATCGGGCCGCGGTG
GATGCGACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGCACGTCAATCGGACCGAACGACACGGCGATCGCCGGGCA
TGCCATTGCCGCCGGTGCCGTGCTGGTGACGAACAGTGTGGGAGAGTTTGAGCGGGTGCCGGGTCTGGTGATGGAAGACT
GGGTTAACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8691.80 Da
• Isoelectric Point: 4.6310

>AT35110 WP_182968128.1 NZ_AP022258:67469-67699 [Klebsiella sp. WP8-S18-ESBL-06]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFLSLEKADPDFMAERGDVVSDEGRVER

Nucleotide

• Length: 231 bp

>AT35110 NZ_AP022258:67469-67699 [Klebsiella sp. WP8-S18-ESBL-06]
ATGAGAACCGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTTCCCCGAGATCTGGATTTTGAAGGAGTGAG
CGAGCTCGAGATCGTCCGGGAGGGGGACAGCATCATTCTGCGTCCCGTCCGCCCGACCTGGAGCTCATTCCTCAGCCTGG
AAAAAGCAGACCCGGACTTTATGGCGGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGGTTGAACGGTGA