35108

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 35168..35694 Replicon plasmid pWP8-S18-ESBL-06_2
Accession NZ_AP022258
Organism Klebsiella sp. WP8-S18-ESBL-06

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H7R76_RS24535 Protein ID WP_000323025.1
Coordinates 35168..35455 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H7R76_RS24540 Protein ID WP_000534858.1
Coordinates 35455..35694 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R76_RS24510 31522..32337 + 816 WP_023338079.1 hypothetical protein -
H7R76_RS24515 32373..32954 + 582 WP_029396071.1 hypothetical protein -
H7R76_RS24520 32966..33475 + 510 WP_023338081.1 hypothetical protein -
H7R76_RS24525 33751..34863 - 1113 WP_001300563.1 IS4-like element IS421 family transposase -
H7R76_RS24530 34941..35096 - 156 WP_001352007.1 type I toxin-antitoxin system toxin HokD -
H7R76_RS24535 35168..35455 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H7R76_RS24540 35455..35694 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H7R76_RS24545 35957..36880 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
H7R76_RS24550 37080..37652 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
H7R76_RS24555 38159..39319 + 1161 WP_000830285.1 IS30-like element ISCfr4 family transposase -
H7R76_RS24560 39784..40137 + 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
H7R76_RS24565 40185..40547 + 363 WP_000783215.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..72104 72104
- inside IScluster/Tn - - 33588..55697 22109


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T35108 WP_000323025.1 NZ_AP022258:c35455-35168 [Klebsiella sp. WP8-S18-ESBL-06]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T35108 NZ_AP022258:c35455-35168 [Klebsiella sp. WP8-S18-ESBL-06]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT35108 WP_000534858.1 NZ_AP022258:c35694-35455 [Klebsiella sp. WP8-S18-ESBL-06]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT35108 NZ_AP022258:c35694-35455 [Klebsiella sp. WP8-S18-ESBL-06]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References