Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 35168..35694 Replicon plasmid pWP8-S18-ESBL-06_2
Accession NZ_AP022258
Organism Klebsiella sp. WP8-S18-ESBL-06

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H7R76_RS24535 Protein ID WP_000323025.1
Coordinates 35168..35455 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H7R76_RS24540 Protein ID WP_000534858.1
Coordinates 35455..35694 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R76_RS24510 31522..32337 + 816 WP_023338079.1 hypothetical protein -
H7R76_RS24515 32373..32954 + 582 WP_029396071.1 hypothetical protein -
H7R76_RS24520 32966..33475 + 510 WP_023338081.1 hypothetical protein -
H7R76_RS24525 33751..34863 - 1113 WP_001300563.1 IS4-like element IS421 family transposase -
H7R76_RS24530 34941..35096 - 156 WP_001352007.1 type I toxin-antitoxin system toxin HokD -
H7R76_RS24535 35168..35455 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H7R76_RS24540 35455..35694 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H7R76_RS24545 35957..36880 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
H7R76_RS24550 37080..37652 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
H7R76_RS24555 38159..39319 + 1161 WP_000830285.1 IS30-like element ISCfr4 family transposase -
H7R76_RS24560 39784..40137 + 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
H7R76_RS24565 40185..40547 + 363 WP_000783215.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..72104 72104
- inside IScluster/Tn - - 33588..55697 22109


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T35108 WP_000323025.1 NZ_AP022258:c35455-35168 [Klebsiella sp. WP8-S18-ESBL-06]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T35108 NZ_AP022258:c35455-35168 [Klebsiella sp. WP8-S18-ESBL-06]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT35108 WP_000534858.1 NZ_AP022258:c35694-35455 [Klebsiella sp. WP8-S18-ESBL-06]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT35108 NZ_AP022258:c35694-35455 [Klebsiella sp. WP8-S18-ESBL-06]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References