Detailed information of TA system TA035107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 83881..84684
• Replicon: plasmid pWP8-S18-ESBL-06_1
• Accession: NZ_AP022257
• Organism: Klebsiella sp. WP8-S18-ESBL-06

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: H7R76_RS24120
• Protein ID: WP_182039154.1
• Coordinates: 84154..84684 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W8E6Q5
• Locus tag: H7R76_RS24115
• Protein ID: WP_022652312.1
• Coordinates: 83881..84150 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R76_RS24090	78987..79589	+	603	Protein_78	hypothetical protein	-
H7R76_RS24095	79623..80396	+	774	Protein_79	IS5 family transposase	-
H7R76_RS24100	80712..80852	+	141	WP_182968095.1	transcriptional regulator	-
H7R76_RS24105	80973..81713	+	741	WP_045282345.1	site-specific integrase	-
H7R76_RS24110	82036..83025	-	990	WP_131619246.1	RepB family plasmid replication initiator protein	-
H7R76_RS24115	83881..84150	+	270	WP_022652312.1	DUF1778 domain-containing protein	Antitoxin
H7R76_RS24120	84154..84684	+	531	WP_182039154.1	GNAT family N-acetyltransferase	Toxin
H7R76_RS24125	84820..85907	+	1088	WP_088569307.1	IS3 family transposase	-
H7R76_RS24130	86084..86809	+	726	WP_000331736.1	MerR family transcriptional regulator	-
H7R76_RS24135	86909..87319	+	411	WP_074526384.1	hydroxyisourate hydrolase	-
H7R76_RS24140	87422..88381	+	960	WP_000208728.1	DUF523 and DUF1722 domain-containing protein	-
H7R76_RS24145	88527..89615	+	1089	WP_000633445.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gnd / rffG / wza	1..128811	128811
	-	inside	IScluster/Tn	-	-	78263..93403	15140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20376.32 Da
• Isoelectric Point: 6.4907

>T35107 WP_182039154.1 NZ_AP022257:84154-84684 [Klebsiella sp. WP8-S18-ESBL-06]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGEILVTHAMKVVYQASQAVGIHGMFVEALNDNAQKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVKDE

Nucleotide

• Length: 531 bp

>T35107 NZ_AP022257:84154-84684 [Klebsiella sp. WP8-S18-ESBL-06]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAATACTTGTGACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGCATACACGGAATGTTCGTGG
AGGCGCTGAACGACAACGCCCAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAGAACTGTAATTCGCTC
TTCTACCCGACAAAATCTATTGAAGAGTTGTTCGAAGTAAAAGATGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10269.70 Da
• Isoelectric Point: 5.7252

>AT35107 WP_022652312.1 NZ_AP022257:83881-84150 [Klebsiella sp. WP8-S18-ESBL-06]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Nucleotide

• Length: 270 bp

>AT35107 NZ_AP022257:83881-84150 [Klebsiella sp. WP8-S18-ESBL-06]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCCTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2Z3XDS5