Detailed information of TA system TA035106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4884231..4884847
• Replicon: chromosome
• Accession: NZ_AP022256
• Organism: Klebsiella sp. WP8-S18-ESBL-06

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H7R76_RS23330
• Protein ID: WP_106929282.1
• Coordinates: 4884231..4884602 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: H7R76_RS23335
• Protein ID: WP_182968073.1
• Coordinates: 4884599..4884847 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R76_RS23315	4881735..4882637	+	903	WP_106929286.1	formate dehydrogenase subunit beta	-
H7R76_RS23320	4882634..4883269	+	636	WP_006179126.1	formate dehydrogenase cytochrome b556 subunit	-
H7R76_RS23325	4883266..4884195	+	930	WP_106929284.1	formate dehydrogenase accessory protein FdhE	-
H7R76_RS23330	4884231..4884602	-	372	WP_106929282.1	type II toxin-antitoxin system VapC family toxin	Toxin
H7R76_RS23335	4884599..4884847	-	249	WP_182968073.1	ribbon-helix-helix domain-containing protein	Antitoxin
H7R76_RS23340	4885061..4886281	-	1221	WP_000343760.1	ISL3-like element ISKox3 family transposase	-
H7R76_RS23345	4886377..4887288	+	912	WP_182966613.1	alpha/beta hydrolase	-
H7R76_RS23350	4887312..4888253	-	942	WP_181358030.1	fatty acid biosynthesis protein FabY	-
H7R76_RS23355	4888298..4888735	-	438	WP_106929276.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H7R76_RS23360	4888732..4889598	-	867	WP_106929274.1	virulence factor BrkB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4873124..4886281	13157
	-	flank	IS/Tn	-	-	4885061..4886281	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14118.53 Da
• Isoelectric Point: 9.5896

>T35106 WP_106929282.1 NZ_AP022256:c4884602-4884231 [Klebsiella sp. WP8-S18-ESBL-06]
MKRPVLFDTNILIDLFSGRMEAKRAMDSYPPQHAISLITWMEVMIGAKRFHQEQRTRIALSAFEIIGVTEEIAEQSVNLR
QEYGMKLPDAIILATAHVHRRELVTRNTKDFARLPGVILPYAL

Nucleotide

• Length: 372 bp

>T35106 NZ_AP022256:c4884602-4884231 [Klebsiella sp. WP8-S18-ESBL-06]
ATGAAAAGACCCGTTCTTTTTGATACCAATATTTTAATCGATCTCTTCAGCGGGCGGATGGAGGCGAAGCGGGCGATGGA
TTCCTACCCTCCGCAACATGCGATAAGCCTGATCACCTGGATGGAGGTCATGATAGGGGCAAAGCGCTTTCATCAGGAGC
AGCGAACGCGAATAGCCTTGAGCGCATTCGAAATTATTGGTGTGACAGAAGAAATTGCTGAGCAGAGTGTGAATCTACGG
CAGGAGTACGGCATGAAGCTGCCCGATGCGATTATTCTGGCCACTGCGCATGTTCATCGCCGGGAACTGGTGACGCGTAA
TACAAAGGATTTTGCGAGGCTTCCGGGCGTGATTTTACCCTACGCGCTGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9715.11 Da
• Isoelectric Point: 4.4777

>AT35106 WP_182968073.1 NZ_AP022256:c4884847-4884599 [Klebsiella sp. WP8-S18-ESBL-06]
MMVERDMGRILLDLSDEVIKRLDILKQQRNLPRAELLREAVEQYLEKQSQAELTISGALGIWQGCEEDGVEYERKLREEW
PR

Nucleotide

• Length: 249 bp

>AT35106 NZ_AP022256:c4884847-4884599 [Klebsiella sp. WP8-S18-ESBL-06]
ATGATGGTGGAAAGGGACATGGGCAGAATACTTCTCGATTTGTCGGACGAGGTGATTAAGCGCCTCGACATACTTAAACA
GCAGCGAAATCTGCCTCGTGCGGAACTGCTGCGTGAAGCCGTTGAGCAATATCTTGAAAAGCAGAGTCAGGCTGAGCTCA
CTATTTCTGGCGCGCTTGGCATCTGGCAAGGGTGTGAAGAAGATGGCGTTGAGTATGAAAGAAAACTTCGGGAAGAGTGG
CCGCGATGA