Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 3866550..3867229 | Replicon | chromosome |
| Accession | NZ_AP022256 | ||
| Organism | Klebsiella sp. WP8-S18-ESBL-06 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | A0A1B7JLK7 |
| Locus tag | H7R76_RS18510 | Protein ID | WP_003031349.1 |
| Coordinates | 3866888..3867229 (+) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | A0A1B7JLJ2 |
| Locus tag | H7R76_RS18505 | Protein ID | WP_003031347.1 |
| Coordinates | 3866550..3866867 (+) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H7R76_RS18465 | 3861677..3862797 | + | 1121 | WP_096147877.1 | IS3-like element ISSen4 family transposase | - |
| H7R76_RS18470 | 3862913..3863362 | + | 450 | WP_032296090.1 | hypothetical protein | - |
| H7R76_RS18475 | 3863359..3863580 | + | 222 | WP_003847740.1 | helix-turn-helix domain-containing protein | - |
| H7R76_RS18480 | 3863715..3864188 | + | 474 | Protein_3624 | hypothetical protein | - |
| H7R76_RS18485 | 3864337..3865317 | - | 981 | WP_068815383.1 | IS5-like element ISKpn26 family transposase | - |
| H7R76_RS18490 | 3865352..3865810 | + | 459 | WP_159359444.1 | antirestriction protein | - |
| H7R76_RS18495 | 3865826..3866302 | + | 477 | WP_003031345.1 | RadC family protein | - |
| H7R76_RS18500 | 3866311..3866532 | + | 222 | WP_003031346.1 | DUF987 domain-containing protein | - |
| H7R76_RS18505 | 3866550..3866867 | + | 318 | WP_003031347.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| H7R76_RS18510 | 3866888..3867229 | + | 342 | WP_003031349.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| H7R76_RS18515 | 3867836..3868195 | + | 360 | WP_182966044.1 | hypothetical protein | - |
| H7R76_RS18520 | 3868188..3868271 | - | 84 | Protein_3632 | plasmid pRiA4b ORF-3 family protein | - |
| H7R76_RS18525 | 3868860..3869237 | - | 378 | WP_072205851.1 | hypothetical protein | - |
| H7R76_RS18530 | 3869643..3869924 | - | 282 | WP_182966046.1 | PerC family transcriptional regulator | - |
| H7R76_RS18535 | 3869936..3870370 | - | 435 | WP_182919194.1 | single-stranded DNA-binding protein | - |
| H7R76_RS18540 | 3870370..3870579 | - | 210 | WP_048293909.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 3858680..3924234 | 65554 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12921.01 Da Isoelectric Point: 9.6543
>T35105 WP_003031349.1 NZ_AP022256:3866888-3867229 [Klebsiella sp. WP8-S18-ESBL-06]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T35105 NZ_AP022256:3866888-3867229 [Klebsiella sp. WP8-S18-ESBL-06]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATAGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATAGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA
Antitoxin
Download Length: 106 a.a. Molecular weight: 12094.74 Da Isoelectric Point: 7.0370
>AT35105 WP_003031347.1 NZ_AP022256:3866550-3866867 [Klebsiella sp. WP8-S18-ESBL-06]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT35105 NZ_AP022256:3866550-3866867 [Klebsiella sp. WP8-S18-ESBL-06]
ATGAGCAAAATCACATGGGGTCTGCAGCGTGATATCACGCCACGTCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATGACCGGCAAGTTCAGTGACATCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACTGGTGAACTCAGCACCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA
ATGAGCAAAATCACATGGGGTCTGCAGCGTGATATCACGCCACGTCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATGACCGGCAAGTTCAGTGACATCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACTGGTGAACTCAGCACCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1B7JLK7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1B7JLJ2 |