Detailed information of TA system TA035104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3708758..3709376
• Replicon: chromosome
• Accession: NZ_AP022256
• Organism: Klebsiella sp. WP8-S18-ESBL-06

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1B7JI95
• Locus tag: H7R76_RS17730
• Protein ID: WP_064547995.1
• Coordinates: 3709158..3709376 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: H7R76_RS17725
• Protein ID: WP_106927922.1
• Coordinates: 3708758..3709132 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R76_RS17715	3703857..3705053	+	1197	WP_106930570.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
H7R76_RS17720	3705076..3708222	+	3147	WP_182929375.1	multidrug efflux RND transporter permease subunit AcrB	-
H7R76_RS17725	3708758..3709132	+	375	WP_106927922.1	Hha toxicity modulator TomB	Antitoxin
H7R76_RS17730	3709158..3709376	+	219	WP_064547995.1	hemolysin expression modulator Hha	Toxin
H7R76_RS17735	3709509..3710066	+	558	WP_182965978.1	maltose O-acetyltransferase	-
H7R76_RS17740	3710169..3710639	+	471	WP_106927926.1	hypothetical protein	-
H7R76_RS17745	3710608..3712065	-	1458	WP_106927928.1	PLP-dependent aminotransferase family protein	-
H7R76_RS17750	3712170..3712868	+	699	WP_106927930.1	GNAT family N-acetyltransferase	-
H7R76_RS17755	3712865..3713005	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
H7R76_RS17760	3713005..3713268	-	264	WP_106927932.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8629.97 Da
• Isoelectric Point: 8.8580

>T35104 WP_064547995.1 NZ_AP022256:3709158-3709376 [Klebsiella sp. WP8-S18-ESBL-06]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSTVWKYIR

Nucleotide

• Length: 219 bp

>T35104 NZ_AP022256:3709158-3709376 [Klebsiella sp. WP8-S18-ESBL-06]
ATGTCTGATAAACCACTAACTAAAACTGATTATTTGATGCGCCTGCGACGCTGCCAGTCTATTGACACGCTCGAACGCGT
AATTGAAAAAAATAAATATGAGCTGTCCGACAATGAACTGGCGGTATTTTACTCAGCGGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAACTCTACGATAAGATCCCTTCAACCGTGTGGAAGTACATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14383.21 Da
• Isoelectric Point: 4.8886

>AT35104 WP_106927922.1 NZ_AP022256:3708758-3709132 [Klebsiella sp. WP8-S18-ESBL-06]
MDEYSPKRHDIAQLKFLCETLYHDCLATLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYDEDNKLIAQIDEYL
DDTFMLFSNYGINSVDLQKWRKSGNKLFRCFVNASRANPVSLSC

Nucleotide

• Length: 375 bp

>AT35104 NZ_AP022256:3708758-3709132 [Klebsiella sp. WP8-S18-ESBL-06]
ATGGACGAATACTCACCAAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGCGAAACCCTGTATCATGATTGTCTTGC
CACGCTCGAAGAGAGTAACCATGGCTGGGTGAACGATCCCACCTCGGCGGTTAACCTGCAGCTTAATGAGCTGATAGAAC
ATATAGCAACGTTCGCACTTAATTATAAAATTAAGTACGACGAAGATAATAAACTGATTGCACAGATTGACGAATATCTG
GATGATACTTTTATGTTATTCAGTAACTACGGCATTAATAGCGTCGACCTGCAAAAATGGCGGAAGTCAGGGAACAAACT
CTTCCGTTGTTTTGTCAATGCGAGCCGCGCCAACCCCGTCAGTCTTTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1B7JI95