Detailed information of TA system TA035090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 374813..375399
• Replicon: chromosome
• Accession: NZ_AP022256
• Organism: Klebsiella sp. WP8-S18-ESBL-06

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: H7R76_RS01780
• Protein ID: WP_182966834.1
• Coordinates: 375031..375399 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: H7R76_RS01775
• Protein ID: WP_181357971.1
• Coordinates: 374813..375034 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R76_RS01750	369868..370977	+	1110	WP_106925084.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
H7R76_RS01755	371032..371958	+	927	WP_106925085.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
H7R76_RS01760	371955..373235	+	1281	WP_106925086.1	branched chain amino acid ABC transporter permease LivM	-
H7R76_RS01765	373232..373999	+	768	WP_182929886.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
H7R76_RS01770	374001..374714	+	714	WP_106925088.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
H7R76_RS01775	374813..375034	+	222	WP_181357971.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H7R76_RS01780	375031..375399	+	369	WP_182966834.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
H7R76_RS01785	375632..376948	+	1317	WP_182966836.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
H7R76_RS01790	377049..377936	+	888	WP_106925091.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
H7R76_RS01795	377933..378778	+	846	WP_106925092.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
H7R76_RS01800	378781..379851	+	1071	WP_182929888.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	363134..380588	17454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (28-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13556.82 Da
• Isoelectric Point: 6.7249

>T35090 WP_182966834.1 NZ_AP022256:375031-375399 [Klebsiella sp. WP8-S18-ESBL-06]
MTLQIISAEEIIQFHDRLLRVTPGVEGMPDPGRAEALMYRVLNQIEYQGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLPTNPDFVAMTVEAAAGQLTLEQIAARLRG

Nucleotide

• Length: 369 bp

>T35090 NZ_AP022256:375031-375399 [Klebsiella sp. WP8-S18-ESBL-06]
ATGACCCTTCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGTTGCTCCGCGTAACGCCTGGCGTAGAGGG
AATGCCCGATCCTGGCCGCGCAGAAGCGCTGATGTACCGGGTGCTCAATCAAATCGAATATCAAGGCGTGACAGATGTCT
GGCTGCTTGCGGCAATGCATCTGCTGGCGATTTCGCGTGGGCATATTTTTAATGACGGAAATAAACGGACGGCATTATTT
ATTACCTTACTGTTTTTAAAGCGTAACGGCATTTCATTACCGACTAATCCTGATTTTGTTGCGATGACGGTTGAGGCTGC
CGCTGGGCAACTCACGCTGGAGCAGATAGCAGCCCGGCTCCGTGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8146.18 Da
• Isoelectric Point: 5.0775

>AT35090 WP_181357971.1 NZ_AP022256:374813-375034 [Klebsiella sp. WP8-S18-ESBL-06]
MRTINYSEARQNLAEVLESAVSGGPVTITRRGHKPAVIISAEEFERYQAARMDDEFAAIMAIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT35090 NZ_AP022256:374813-375034 [Klebsiella sp. WP8-S18-ESBL-06]
ATGCGTACGATTAACTACAGTGAAGCGCGGCAGAATCTGGCGGAGGTACTGGAAAGTGCCGTCAGCGGGGGGCCTGTCAC
CATTACGCGCCGAGGGCATAAGCCTGCGGTGATTATCAGCGCGGAAGAATTTGAGCGTTATCAGGCGGCCAGAATGGATG
ATGAATTCGCGGCAATCATGGCGATACATGGTAATGAAATCAGGGAGCTGGCAGATAAATGA