Detailed information of TA system TA035064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 1153..1718
• Replicon: plasmid pWP8-S18-CRE-01_3
• Accession: NZ_AP022244
• Organism: Aeromonas caviae strain WP8-S18-CRE-01

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: H7R78_RS22680
• Protein ID: WP_130627593.1
• Coordinates: 1428..1718 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H7R78_RS22675
• Protein ID: WP_130627592.1
• Coordinates: 1153..1440 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R78_RS22665	101..298	-	198	WP_130627590.1	chromosome partitioning protein ParB	-
H7R78_RS22670	319..948	-	630	WP_130627591.1	AAA family ATPase	-
H7R78_RS22675	1153..1440	+	288	WP_130627592.1	CopG family ribbon-helix-helix protein	Antitoxin
H7R78_RS22680	1428..1718	+	291	WP_130627593.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R78_RS22685	2130..2384	+	255	WP_130627594.1	DUF4258 domain-containing protein	-
H7R78_RS22690	2466..2966	+	501	WP_130627595.1	hypothetical protein	-
H7R78_RS22695	2963..3352	+	390	WP_130627596.1	hypothetical protein	-
H7R78_RS22700	3371..4318	+	948	WP_130627586.1	hypothetical protein	-
H7R78_RS22705	5336..5584	+	249	WP_130627587.1	hypothetical protein	-
H7R78_RS22710	5584..6468	+	885	WP_130627588.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..8098	8098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (8-45)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10904.82 Da
• Isoelectric Point: 10.6871

>T35064 WP_130627593.1 NZ_AP022244:1428-1718 [Aeromonas caviae]
MPPVIFAPAALRDLQRLREFLRPKSPLVAQRAATTIQLSLRQLAAQPLMGRPIEGLPEAFREWVIPFGDSGYLARYRIEP
DVIVVLAVRHQREVGY

Nucleotide

• Length: 291 bp

>T35064 NZ_AP022244:1428-1718 [Aeromonas caviae]
ATGCCACCAGTGATCTTTGCCCCTGCAGCCCTGCGAGATCTGCAACGCCTACGTGAATTCCTGCGCCCTAAAAGTCCGCT
GGTAGCACAGCGAGCCGCAACAACAATCCAGCTGTCTCTACGCCAGCTCGCAGCCCAACCCTTAATGGGCCGCCCCATTG
AAGGACTGCCAGAGGCGTTTCGTGAATGGGTGATCCCTTTTGGAGACAGTGGCTATTTAGCTCGTTATCGCATTGAACCG
GACGTTATTGTGGTGCTCGCAGTCCGCCATCAACGCGAAGTGGGCTACTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11063.15 Da
• Isoelectric Point: 4.7091

>AT35064 WP_130627592.1 NZ_AP022244:1153-1440 [Aeromonas caviae]
MPTQSKPVSIKLDADTQERIKRLATSRERSAHWLMREAISQYVEREELRETFHRDTLEAWQEFQETGQYATAQDVDQWLS
SWGTDAEGAPPACHQ

Nucleotide

• Length: 288 bp

>AT35064 NZ_AP022244:1153-1440 [Aeromonas caviae]
ATGCCCACGCAATCCAAACCGGTTTCGATCAAACTCGACGCCGACACCCAGGAACGCATCAAACGCCTGGCAACCTCCCG
CGAACGCTCGGCCCATTGGCTCATGCGTGAAGCCATCAGCCAGTATGTCGAGCGCGAAGAACTGCGCGAGACCTTCCACC
GTGACACTCTGGAGGCCTGGCAGGAGTTCCAGGAGACCGGCCAGTATGCCACCGCCCAAGATGTGGATCAGTGGCTAAGC
TCTTGGGGCACTGATGCCGAAGGAGCGCCACCGGCATGCCACCAGTGA