Detailed information of TA system TA035063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 2541..3234
• Replicon: plasmid pWP8-S18-CRE-01_2
• Accession: NZ_AP022243
• Organism: Aeromonas caviae strain WP8-S18-CRE-01

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: H7R78_RS22360
• Protein ID: WP_000182276.1
• Coordinates: 2878..3234 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: H7R78_RS22355
• Protein ID: WP_001172026.1
• Coordinates: 2541..2876 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R78_RS22330	101..1015	-	915	WP_001736514.1	HD domain-containing protein	-
H7R78_RS22335	1062..1346	-	285	WP_130627582.1	DUF3391 domain-containing protein	-
H7R78_RS22340	1464..1844	-	381	WP_001054412.1	hypothetical protein	-
H7R78_RS22345	1841..2167	-	327	WP_000091614.1	hypothetical protein	-
H7R78_RS22350	2191..2526	-	336	WP_000741275.1	hypothetical protein	-
H7R78_RS22355	2541..2876	-	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
H7R78_RS22360	2878..3234	-	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R78_RS22365	3426..4028	+	603	WP_010465829.1	recombinase family protein	-
H7R78_RS22370	4012..7041	+	3030	WP_032432545.1	Tn3 family transposase	-
H7R78_RS22375	6998..7933	-	936	WP_130627585.1	replication protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mph(A) / blaKPC-2	-	1..53629	53629

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T35063 WP_000182276.1 NZ_AP022243:c3234-2878 [Aeromonas caviae]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T35063 NZ_AP022243:c3234-2878 [Aeromonas caviae]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT35063 WP_001172026.1 NZ_AP022243:c2876-2541 [Aeromonas caviae]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT35063 NZ_AP022243:c2876-2541 [Aeromonas caviae]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7