Detailed information of TA system TA035049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 33425..33984
• Replicon: plasmid pWP8-S17-ESBL-12_2
• Accession: NZ_AP022224
• Organism: Escherichia coli strain WP8-S17-ESBL-12

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: H7R20_RS24750
• Protein ID: WP_000421257.1
• Coordinates: 33425..33700 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: H7R20_RS24755
• Protein ID: WP_001178087.1
• Coordinates: 33700..33984 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R20_RS24720	28673..29299	+	627	WP_000587689.1	ParA family plasmid-partitioning AAA ATPase	-
H7R20_RS24725	29296..29598	+	303	WP_001245884.1	hypothetical protein	-
H7R20_RS24730	30141..31487	-	1347	WP_001132022.1	hypothetical protein	-
H7R20_RS24735	31841..32443	+	603	WP_000517694.1	hypothetical protein	-
H7R20_RS24740	32581..32847	+	267	WP_000627831.1	hypothetical protein	-
H7R20_RS24745	33038..33319	-	282	WP_001105066.1	hypothetical protein	-
H7R20_RS24750	33425..33700	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R20_RS24755	33700..33984	-	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
H7R20_RS24760	34887..35759	-	873	WP_000729438.1	incFII family plasmid replication initiator RepA	-
H7R20_RS24765	36462..37103	+	642	WP_021515453.1	hypothetical protein	-
H7R20_RS24770	37243..37905	+	663	WP_005082527.1	hypothetical protein	-
H7R20_RS24775	38172..38315	+	144	WP_000176500.1	hypothetical protein	-
H7R20_RS24780	38396..38638	+	243	WP_021515452.1	PilI type IV pilus biogenesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..87171	87171

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T35049 WP_000421257.1 NZ_AP022224:c33700-33425 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T35049 NZ_AP022224:c33700-33425 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT35049 WP_001178087.1 NZ_AP022224:c33984-33700 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT35049 NZ_AP022224:c33984-33700 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: U9Y625