Detailed information of TA system TA035040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 48047..48572
• Replicon: plasmid pWP8-S17-ESBL-12_1
• Accession: NZ_AP022223
• Organism: Escherichia coli strain WP8-S17-ESBL-12

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H7R20_RS23970
• Protein ID: WP_182924578.1
• Coordinates: 48267..48572 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: H9TJP1
• Locus tag: H7R20_RS23965
• Protein ID: WP_000813630.1
• Coordinates: 48047..48265 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R20_RS23930	43088..43648	-	561	WP_021532377.1	recombinase family protein	-
H7R20_RS23935	43699..44403	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H7R20_RS23940	44602..44880	-	279	WP_004217866.1	DUF3330 domain-containing protein	-
H7R20_RS23945	44944..45648	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
H7R20_RS23950	45702..46769	-	1068	Protein_53	AAA family ATPase	-
H7R20_RS23955	46844..47260	-	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	-
H7R20_RS23960	47257..47487	-	231	WP_001261286.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
H7R20_RS23965	48047..48265	+	219	WP_000813630.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
H7R20_RS23970	48267..48572	+	306	WP_182924578.1	type II toxin-antitoxin system toxin CcdB	Toxin
H7R20_RS23975	48573..49379	+	807	WP_000016970.1	site-specific integrase	-
H7R20_RS23980	50101..50856	+	756	WP_000852146.1	replication initiation protein RepE	-
H7R20_RS23985	51444..52610	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / catA1 / blaTEM-1B	-	1..139020	139020

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11660.43 Da
• Isoelectric Point: 6.4674

>T35040 WP_182924578.1 NZ_AP022223:48267-48572 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLVFWGI

Nucleotide

• Length: 306 bp

>T35040 NZ_AP022223:48267-48572 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGGTGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8354.27 Da
• Isoelectric Point: 4.5203

>AT35040 WP_000813630.1 NZ_AP022223:48047-48265 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT35040 NZ_AP022223:48047-48265 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CEF5