35039

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 46844..47487 Replicon plasmid pWP8-S17-ESBL-12_1
Accession NZ_AP022223
Organism Escherichia coli strain WP8-S17-ESBL-12

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag H7R20_RS23955 Protein ID WP_001034044.1
Coordinates 46844..47260 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag H7R20_RS23960 Protein ID WP_001261286.1
Coordinates 47257..47487 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R20_RS23930 43088..43648 - 561 WP_021532377.1 recombinase family protein -
H7R20_RS23935 43699..44403 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
H7R20_RS23940 44602..44880 - 279 WP_004217866.1 DUF3330 domain-containing protein -
H7R20_RS23945 44944..45648 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
H7R20_RS23950 45702..46769 - 1068 Protein_53 AAA family ATPase -
H7R20_RS23955 46844..47260 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R20_RS23960 47257..47487 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R20_RS23965 48047..48265 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
H7R20_RS23970 48267..48572 + 306 WP_182924578.1 type II toxin-antitoxin system toxin CcdB -
H7R20_RS23975 48573..49379 + 807 WP_000016970.1 site-specific integrase -
H7R20_RS23980 50101..50856 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / catA1 / blaTEM-1B - 1..139020 139020


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T35039 WP_001034044.1 NZ_AP022223:c47260-46844 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T35039 NZ_AP022223:c47260-46844 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT35039 WP_001261286.1 NZ_AP022223:c47487-47257 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT35039 NZ_AP022223:c47487-47257 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References