Detailed information of TA system TA035037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4280255..4281069
• Replicon: chromosome
• Accession: NZ_AP022222
• Organism: Escherichia coli strain WP8-S17-ESBL-12

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: H7R20_RS20400
• Protein ID: WP_001054376.1
• Coordinates: 4280255..4280512 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: H7R20_RS20405
• Protein ID: WP_001309181.1
• Coordinates: 4280524..4281069 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R20_RS20380	4275543..4276649	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
H7R20_RS20385	4276714..4277694	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
H7R20_RS20390	4278277..4279517	-	1241	Protein_3989	DNA helicase	-
H7R20_RS20395	4279633..4279878	+	246	Protein_3990	GNAT family N-acetyltransferase	-
H7R20_RS20400	4280255..4280512	+	258	WP_001054376.1	hypothetical protein	Toxin
H7R20_RS20405	4280524..4281069	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
H7R20_RS20410	4281125..4281871	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
H7R20_RS20415	4282040..4282258	+	219	Protein_3994	hypothetical protein	-
H7R20_RS25525	4282296..4282412	+	117	Protein_3995	VOC family protein	-
H7R20_RS20420	4282657..4283778	+	1122	WP_000010829.1	M42 family metallopeptidase	-
H7R20_RS20425	4283775..4284053	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
H7R20_RS20430	4284065..4285378	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4272749..4289293	16544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T35037 WP_001054376.1 NZ_AP022222:4280255-4280512 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T35037 NZ_AP022222:4280255-4280512 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT35037 WP_001309181.1 NZ_AP022222:4280524-4281069 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT35037 NZ_AP022222:4280524-4281069 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8