Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2622966..2623604 Replicon chromosome
Accession NZ_AP022222
Organism Escherichia coli strain WP8-S17-ESBL-12

Toxin (Protein)


Gene name hicA Uniprot ID B7N4J4
Locus tag H7R20_RS12490 Protein ID WP_000813797.1
Coordinates 2623428..2623604 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H7R20_RS12485 Protein ID WP_076611057.1
Coordinates 2622966..2623382 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R20_RS12465 2618118..2619059 - 942 WP_001251335.1 ABC transporter permease -
H7R20_RS12470 2619060..2620073 - 1014 WP_000220413.1 ABC transporter ATP-binding protein -
H7R20_RS12475 2620091..2621236 - 1146 WP_000047447.1 ABC transporter substrate-binding protein -
H7R20_RS12480 2621481..2622887 - 1407 WP_000760613.1 PLP-dependent aminotransferase family protein -
H7R20_RS12485 2622966..2623382 - 417 WP_076611057.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H7R20_RS12490 2623428..2623604 - 177 WP_000813797.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H7R20_RS12495 2623826..2624056 + 231 WP_000494244.1 YncJ family protein -
H7R20_RS12500 2624148..2626109 - 1962 WP_001309488.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H7R20_RS12505 2626182..2626718 - 537 WP_000429161.1 DNA-binding transcriptional regulator SutR -
H7R20_RS12510 2626771..2627982 + 1212 WP_001323162.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6724.76 Da        Isoelectric Point: 11.5666

>T35031 WP_000813797.1 NZ_AP022222:c2623604-2623428 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T35031 NZ_AP022222:c2623604-2623428 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15251.65 Da        Isoelectric Point: 4.5908

>AT35031 WP_076611057.1 NZ_AP022222:c2623382-2622966 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT35031 NZ_AP022222:c2623382-2622966 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J715

Antitoxin

Source ID Structure

References