Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1974255..1975086 Replicon chromosome
Accession NZ_AP022222
Organism Escherichia coli strain WP8-S17-ESBL-12

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag H7R20_RS09310 Protein ID WP_000854814.1
Coordinates 1974255..1974629 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag H7R20_RS09315 Protein ID WP_001285585.1
Coordinates 1974718..1975086 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R20_RS09270 1969651..1970817 + 1167 WP_000830150.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
H7R20_RS09275 1970936..1971409 + 474 WP_001105389.1 DNA gyrase inhibitor SbmC -
H7R20_RS09280 1971607..1972665 + 1059 WP_001200891.1 FUSC family protein -
H7R20_RS09285 1972837..1973166 + 330 WP_000450409.1 DUF496 family protein -
H7R20_RS09290 1973267..1973449 - 183 WP_001016348.1 hypothetical protein -
H7R20_RS09295 1973521..1973649 + 129 Protein_1820 transposase domain-containing protein -
H7R20_RS09300 1973938..1974051 - 114 WP_001161660.1 DUF957 domain-containing protein -
H7R20_RS09305 1974064..1974258 - 195 WP_000988600.1 hypothetical protein -
H7R20_RS09310 1974255..1974629 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
H7R20_RS09315 1974718..1975086 - 369 WP_001285585.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
H7R20_RS09320 1975160..1975381 - 222 WP_000692323.1 DUF987 domain-containing protein -
H7R20_RS09325 1975444..1975920 - 477 WP_001186726.1 RadC family protein -
H7R20_RS09330 1975936..1976415 - 480 WP_000860087.1 antirestriction protein -
H7R20_RS09335 1976497..1977315 - 819 WP_001234629.1 DUF945 domain-containing protein -
H7R20_RS09340 1977415..1977648 - 234 WP_001119729.1 DUF905 family protein -
H7R20_RS09345 1977727..1978182 - 456 WP_000581504.1 hypothetical protein -
H7R20_RS09350 1978258..1979982 - 1725 Protein_1831 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T35020 WP_000854814.1 NZ_AP022222:c1974629-1974255 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T35020 NZ_AP022222:c1974629-1974255 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT35020 WP_001285585.1 NZ_AP022222:c1975086-1974718 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT35020 NZ_AP022222:c1975086-1974718 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References