Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 943206..943860 Replicon chromosome
Accession NZ_AP022222
Organism Escherichia coli strain WP8-S17-ESBL-12

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag H7R20_RS04555 Protein ID WP_000244769.1
Coordinates 943453..943860 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H7R20_RS04550 Protein ID WP_000354046.1
Coordinates 943206..943472 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R20_RS04530 939294..940727 - 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -
H7R20_RS04535 940772..941083 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
H7R20_RS04540 941247..941906 + 660 WP_000250274.1 hemolysin III family protein -
H7R20_RS04545 941983..942963 - 981 WP_000886049.1 tRNA-modifying protein YgfZ -
H7R20_RS04550 943206..943472 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H7R20_RS04555 943453..943860 + 408 WP_000244769.1 protein YgfX Toxin
H7R20_RS04560 943900..944421 - 522 WP_001055874.1 flavodoxin FldB -
H7R20_RS04565 944533..945429 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
H7R20_RS04570 945454..946164 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H7R20_RS04575 946170..947903 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T35019 WP_000244769.1 NZ_AP022222:943453-943860 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T35019 NZ_AP022222:943453-943860 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGAAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT35019 WP_000354046.1 NZ_AP022222:943206-943472 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT35019 NZ_AP022222:943206-943472 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACCCGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References