Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4501030..4501527 Replicon chromosome
Accession NZ_AP022221
Organism Escherichia coli strain WP8-S17-ESBL-08

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H7K50_RS21390 Protein ID WP_001173343.1
Coordinates 4501030..4501203 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H7K50_RS21395 Protein ID WP_000398619.1
Coordinates 4501231..4501527 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7K50_RS21380 4497700..4499157 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
H7K50_RS21385 4499394..4500911 + 1518 WP_001550472.1 lysine--tRNA ligase -
H7K50_RS21390 4501030..4501203 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H7K50_RS21395 4501231..4501527 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H7K50_RS21400 4501756..4502028 - 273 WP_000405647.1 N-acetyltransferase -
H7K50_RS21405 4502040..4502270 - 231 WP_001550470.1 (4Fe-4S)-binding protein -
H7K50_RS21410 4502450..4504081 + 1632 WP_001216477.1 sensor histidine kinase -
H7K50_RS21415 4504078..4504797 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T35012 WP_001173343.1 NZ_AP022221:c4501203-4501030 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T35012 NZ_AP022221:c4501203-4501030 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT35012 WP_000398619.1 NZ_AP022221:c4501527-4501231 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT35012 NZ_AP022221:c4501527-4501231 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References