Detailed information of TA system TA035009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4285081..4285901
• Replicon: chromosome
• Accession: NZ_AP022221
• Organism: Escherichia coli strain WP8-S17-ESBL-08

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: A0A0V9MKL8
• Locus tag: H7K50_RS20295
• Protein ID: WP_001550554.1
• Coordinates: 4285081..4285338 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: H7K50_RS20300
• Protein ID: WP_001519303.1
• Coordinates: 4285350..4285901 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7K50_RS20275	4280367..4281473	+	1107	WP_001519308.1	N-acetylneuraminate epimerase	-
H7K50_RS20280	4281538..4282518	+	981	WP_001519307.1	transposase	-
H7K50_RS20285	4283101..4284342	-	1242	Protein_3964	DNA helicase	-
H7K50_RS20290	4284405..4284704	+	300	WP_001519304.1	GNAT family N-acetyltransferase	-
H7K50_RS20295	4285081..4285338	+	258	WP_001550554.1	hypothetical protein	Toxin
H7K50_RS20300	4285350..4285901	+	552	WP_001519303.1	N-acetyltransferase	Antitoxin
H7K50_RS20305	4285953..4286114	+	162	WP_001519302.1	hypothetical protein	-
H7K50_RS20310	4286151..4286699	+	549	WP_001519301.1	hypothetical protein	-
H7K50_RS20315	4286826..4287086	+	261	WP_000084876.1	hypothetical protein	-
H7K50_RS23700	4287124..4287240	+	117	Protein_3971	VOC family protein	-
H7K50_RS20320	4287485..4288606	+	1122	WP_000010833.1	M42 family metallopeptidase	-
H7K50_RS20325	4288603..4288881	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
H7K50_RS20330	4288893..4290206	+	1314	WP_001519300.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4277565..4294121	16556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9744.33 Da
• Isoelectric Point: 11.0090

>T35009 WP_001550554.1 NZ_AP022221:4285081-4285338 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKPVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T35009 NZ_AP022221:4285081-4285338 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGCCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20463.61 Da
• Isoelectric Point: 6.8758

>AT35009 WP_001519303.1 NZ_AP022221:4285350-4285901 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT35009 NZ_AP022221:4285350-4285901 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCGACCTTCAAAGGAGAAATGGATTCACCGCTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: A0A0V9MKL8