Detailed information of TA system TA035008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4228870..4229378
• Replicon: chromosome
• Accession: NZ_AP022221
• Organism: Escherichia coli strain WP8-S17-ESBL-08

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: H7K50_RS20020
• Protein ID: WP_001534843.1
• Coordinates: 4229169..4229378 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: H7K50_RS20015
• Protein ID: WP_000063161.1
• Coordinates: 4228870..4229172 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7K50_RS20000	4225398..4227548	+	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-
H7K50_RS20005	4227666..4227869	+	204	WP_001534846.1	YbdD/YjiX family protein	-
H7K50_RS20010	4227880..4228836	+	957	WP_001534845.1	GTPase	-
H7K50_RS20015	4228870..4229172	-	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
H7K50_RS20020	4229169..4229378	-	210	WP_001534843.1	BrnT family toxin	Toxin
H7K50_RS20025	4229474..4230640	+	1167	WP_001534841.1	restriction endonuclease	-
H7K50_RS20030	4230704..4232323	+	1620	WP_001550561.1	type I restriction-modification system subunit M	-
H7K50_RS20035	4232313..4233623	+	1311	WP_001534837.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T35008 WP_001534843.1 NZ_AP022221:c4229378-4229169 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T35008 NZ_AP022221:c4229378-4229169 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT35008 WP_000063161.1 NZ_AP022221:c4229172-4228870 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT35008 NZ_AP022221:c4229172-4228870 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3