Detailed information of TA system TA035004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3265371..3266076
• Replicon: chromosome
• Accession: NZ_AP022221
• Organism: Escherichia coli strain WP8-S17-ESBL-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: H7K50_RS15625
• Protein ID: WP_000539521.1
• Coordinates: 3265371..3265757 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H7K50_RS15630
• Protein ID: WP_001280945.1
• Coordinates: 3265747..3266076 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7K50_RS15605	3261375..3262001	+	627	WP_001295292.1	glutathione S-transferase GstB	-
H7K50_RS15610	3261998..3263113	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
H7K50_RS15615	3263224..3263607	-	384	WP_000497137.1	biofilm formation regulator BssR	-
H7K50_RS15620	3263820..3265145	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
H7K50_RS15625	3265371..3265757	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7K50_RS15630	3265747..3266076	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
H7K50_RS15635	3266146..3267474	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
H7K50_RS15640	3267482..3269830	-	2349	WP_001550886.1	EAL domain-containing protein	-
H7K50_RS15645	3270008..3270919	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T35004 WP_000539521.1 NZ_AP022221:3265371-3265757 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T35004 NZ_AP022221:3265371-3265757 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT35004 WP_001280945.1 NZ_AP022221:3265747-3266076 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT35004 NZ_AP022221:3265747-3266076 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6