Detailed information of TA system TA035002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2633313..2633951
• Replicon: chromosome
• Accession: NZ_AP022221
• Organism: Escherichia coli strain WP8-S17-ESBL-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: H7K50_RS12525
• Protein ID: WP_000813795.1
• Coordinates: 2633775..2633951 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H7K50_RS12520
• Protein ID: WP_076797675.1
• Coordinates: 2633313..2633729 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7K50_RS12500	2628465..2629406	-	942	WP_001251315.1	ABC transporter permease	-
H7K50_RS12505	2629407..2630420	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
H7K50_RS12510	2630438..2631583	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
H7K50_RS12515	2631828..2633234	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
H7K50_RS12520	2633313..2633729	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H7K50_RS12525	2633775..2633951	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H7K50_RS12530	2634173..2634403	+	231	WP_023910283.1	YncJ family protein	-
H7K50_RS12535	2634495..2636456	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H7K50_RS12540	2636529..2637065	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
H7K50_RS12545	2637118..2638329	+	1212	WP_113365709.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T35002 WP_000813795.1 NZ_AP022221:c2633951-2633775 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T35002 NZ_AP022221:c2633951-2633775 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT35002 WP_076797675.1 NZ_AP022221:c2633729-2633313 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT35002 NZ_AP022221:c2633729-2633313 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1