Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 101..623 Replicon plasmid pWP8-S17-ESBL-05_3
Accession NZ_AP022218
Organism Escherichia coli strain WP8-S17-ESBL-05

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag H7R13_RS23990 Protein ID WP_000220560.1
Coordinates 342..623 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag H7R13_RS23985 Protein ID WP_000121743.1
Coordinates 101..352 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R13_RS23985 101..352 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
H7R13_RS23990 342..623 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R13_RS23995 769..1092 + 324 WP_001181903.1 hypothetical protein -
H7R13_RS24000 1137..1382 + 246 WP_000356546.1 hypothetical protein -
H7R13_RS24005 1372..1587 + 216 WP_001180116.1 hypothetical protein -
H7R13_RS24010 1680..2009 + 330 WP_000866648.1 hypothetical protein -
H7R13_RS24015 2296..2445 + 150 WP_000003880.1 hypothetical protein -
H7R13_RS24020 2458..2730 + 273 WP_000160399.1 hypothetical protein -
H7R13_RS24025 3057..3602 + 546 WP_038976855.1 hypothetical protein -
H7R13_RS24030 3605..4762 + 1158 WP_000538023.1 hypothetical protein -
H7R13_RS24035 5123..5614 + 492 WP_000872475.1 transcription termination factor NusG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / blaCTX-M-55 / dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / qnrS1 / tet(A) - 1..53216 53216


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T34984 WP_000220560.1 NZ_AP022218:342-623 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T34984 NZ_AP022218:342-623 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT34984 WP_000121743.1 NZ_AP022218:101-352 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT34984 NZ_AP022218:101-352 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References