Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 77951..78552 Replicon plasmid pWP8-S17-ESBL-05_2
Accession NZ_AP022217
Organism Escherichia coli strain WP8-S17-ESBL-05

Toxin (Protein)


Gene name doc Uniprot ID A0A731GHK2
Locus tag H7R13_RS23885 Protein ID WP_001216038.1
Coordinates 78172..78552 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag H7R13_RS23880 Protein ID WP_001190712.1
Coordinates 77951..78172 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R13_RS23825 73375..73578 + 204 WP_000158003.1 hypothetical protein -
H7R13_RS23830 73571..73810 + 240 WP_000476201.1 hypothetical protein -
H7R13_RS23835 73807..74532 + 726 WP_182913716.1 ead/Ea22-like family protein -
H7R13_RS23840 74534..74728 + 195 WP_000224731.1 hypothetical protein -
H7R13_RS23845 74730..74939 + 210 WP_000951712.1 hypothetical protein -
H7R13_RS23850 74936..76102 + 1167 WP_182913717.1 hypothetical protein -
H7R13_RS23855 76086..76370 + 285 WP_001142397.1 hypothetical protein -
H7R13_RS23860 76355..76564 - 210 WP_001344848.1 hypothetical protein -
H7R13_RS23865 76738..76884 + 147 WP_001443773.1 hypothetical protein -
H7R13_RS23870 76896..77285 + 390 WP_000506729.1 DNA repair protein -
H7R13_RS23875 77420..77842 + 423 WP_182913718.1 hypothetical protein -
H7R13_RS23880 77951..78172 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H7R13_RS23885 78172..78552 + 381 WP_001216038.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
H7R13_RS23890 78557..78754 + 198 WP_000113017.1 hypothetical protein -
H7R13_RS23895 78787..79563 - 777 WP_000432096.1 hypothetical protein -
H7R13_RS23900 79570..80073 - 504 WP_182913719.1 DUF2829 domain-containing protein -
H7R13_RS23905 80261..80755 - 495 WP_000640907.1 dUTP diphosphatase -
H7R13_RS23910 80752..81228 - 477 WP_000861175.1 hypothetical protein -
H7R13_RS23915 81225..81533 - 309 WP_000349257.1 hypothetical protein -
H7R13_RS23920 81643..82671 - 1029 WP_001292235.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..92332 92332


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13574.27 Da        Isoelectric Point: 5.1514

>T34983 WP_001216038.1 NZ_AP022217:78172-78552 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEISDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T34983 NZ_AP022217:78172-78552 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGTGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCAGCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT34983 WP_001190712.1 NZ_AP022217:77951-78172 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT34983 NZ_AP022217:77951-78172 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTTAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CCGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A731GHK2

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References