Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1307338..1307963 Replicon chromosome
Accession NZ_AP022215
Organism Escherichia coli strain WP8-S17-ESBL-05

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag H7R13_RS06205 Protein ID WP_000911329.1
Coordinates 1307565..1307963 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag H7R13_RS06200 Protein ID WP_000450524.1
Coordinates 1307338..1307565 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R13_RS06175 1303140..1303610 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
H7R13_RS06180 1303610..1304182 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
H7R13_RS06185 1304328..1305206 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
H7R13_RS06190 1305223..1306257 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
H7R13_RS06195 1306470..1307183 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
H7R13_RS06200 1307338..1307565 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
H7R13_RS06205 1307565..1307963 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
H7R13_RS06210 1308110..1308973 + 864 WP_001267498.1 neutral zinc metallopeptidase -
H7R13_RS06215 1308988..1311003 + 2016 WP_000829393.1 tRNA cytosine(34) acetyltransferase TmcA -
H7R13_RS06220 1311077..1311775 + 699 WP_000679812.1 esterase -
H7R13_RS06225 1311885..1312085 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T34958 WP_000911329.1 NZ_AP022215:1307565-1307963 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T34958 NZ_AP022215:1307565-1307963 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT34958 WP_000450524.1 NZ_AP022215:1307338-1307565 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT34958 NZ_AP022215:1307338-1307565 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References