Detailed information of TA system TA034950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4770177..4770870
• Replicon: chromosome
• Accession: NZ_AP022213
• Organism: Pseudomonas otitidis strain WP8-S17-CRE-03

Toxin (Protein)

• Gene name: tad
• Uniprot ID: N2IHR9
• Locus tag: H7K70_RS22190
• Protein ID: WP_003151133.1
• Coordinates: 4770493..4770870 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: H7K70_RS22185
• Protein ID: WP_001172026.1
• Coordinates: 4770177..4770512 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7K70_RS22155	4767032..4767514	-	483	WP_089030249.1	recombinase family protein	-
H7K70_RS22160	4767688..4768602	-	915	WP_001736514.1	HD domain-containing protein	-
H7K70_RS22165	4768641..4768982	-	342	WP_182850794.1	DUF3391 domain-containing protein	-
H7K70_RS22170	4769100..4769480	-	381	WP_001054412.1	hypothetical protein	-
H7K70_RS22175	4769477..4769803	-	327	WP_089030250.1	hypothetical protein	-
H7K70_RS22180	4769827..4770162	-	336	WP_000741275.1	hypothetical protein	-
H7K70_RS22185	4770177..4770512	-	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
H7K70_RS22190	4770493..4770870	-	378	WP_003151133.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7K70_RS22195	4771062..4771664	+	603	WP_010465829.1	recombinase family protein	-
H7K70_RS22200	4771648..4774677	+	3030	WP_010799689.1	Tn3 family transposase	-
H7K70_RS22205	4774713..4775030	+	318	Protein_4384	FCD domain-containing protein	-
H7K70_RS22210	4775112..4775702	+	591	WP_003465041.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4736458..4770870	34412
	-	inside	IScluster/Tn	-	-	4764062..4774677	10615

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13705.78 Da
• Isoelectric Point: 9.4693

>T34950 WP_003151133.1 NZ_AP022213:c4770870-4770493 [Pseudomonas otitidis]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T34950 NZ_AP022213:c4770870-4770493 [Pseudomonas otitidis]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT34950 WP_001172026.1 NZ_AP022213:c4770512-4770177 [Pseudomonas otitidis]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT34950 NZ_AP022213:c4770512-4770177 [Pseudomonas otitidis]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A024ELN5

Antitoxin

• AlphaFold DB: A0A024EKI7