Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 184642..185167 Replicon plasmid pWP7-S18-ESBL-09_1
Accession NZ_AP022208
Organism Escherichia coli strain WP7-S18-ESBL-09

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H7R37_RS26150 Protein ID WP_001159871.1
Coordinates 184642..184947 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H7R37_RS26155 Protein ID WP_000813634.1
Coordinates 184949..185167 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R37_RS26115 180069..180866 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
H7R37_RS26120 180928..181179 + 252 Protein_233 DUF4113 domain-containing protein -
H7R37_RS26125 181184..181576 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
H7R37_RS26130 181581..182552 - 972 WP_001103694.1 hypothetical protein -
H7R37_RS26135 182781..183425 + 645 WP_000633911.1 AAA family ATPase -
H7R37_RS26140 183419..183694 + 276 WP_000239529.1 hypothetical protein -
H7R37_RS26145 183832..184641 - 810 WP_000016979.1 site-specific integrase -
H7R37_RS26150 184642..184947 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R37_RS26155 184949..185167 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R37_RS26160 187489..188130 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aac(3)-IId / dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) senB 1..214586 214586


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T34947 WP_001159871.1 NZ_AP022208:c184947-184642 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34947 NZ_AP022208:c184947-184642 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT34947 WP_000813634.1 NZ_AP022208:c185167-184949 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34947 NZ_AP022208:c185167-184949 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References