Detailed information of TA system TA034930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4344653..4345140
• Replicon: chromosome
• Accession: NZ_AP022207
• Organism: Escherichia coli strain WP7-S18-ESBL-09

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H7R37_RS20910
• Protein ID: WP_001520461.1
• Coordinates: 4344653..4344904 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: H7R37_RS20915
• Protein ID: WP_001520458.1
• Coordinates: 4344907..4345140 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R37_RS20885	4340265..4341551	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
H7R37_RS20890	4341551..4342540	+	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
H7R37_RS20895	4342537..4343358	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
H7R37_RS20900	4343361..4343738	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
H7R37_RS20905	4343745..4344593	+	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
H7R37_RS20910	4344653..4344904	-	252	WP_001520461.1	CcdB family protein	Toxin
H7R37_RS20915	4344907..4345140	-	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H7R37_RS20920	4345226..4345705	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
H7R37_RS20925	4345897..4347759	-	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
H7R37_RS20930	4347752..4348282	-	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
H7R37_RS20935	4348390..4349721	-	1332	WP_001520455.1	MFS transporter	-
H7R37_RS20940	4349780..4350067	-	288	WP_000203747.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T34930 WP_001520461.1 NZ_AP022207:c4344904-4344653 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T34930 NZ_AP022207:c4344904-4344653 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATTCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT34930 WP_001520458.1 NZ_AP022207:c4345140-4344907 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT34930 NZ_AP022207:c4345140-4344907 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTATGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4