Detailed information of TA system TA034923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2557084..2557610
• Replicon: chromosome
• Accession: NZ_AP022207
• Organism: Escherichia coli strain WP7-S18-ESBL-09

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: H7R37_RS12330
• Protein ID: WP_000323025.1
• Coordinates: 2557323..2557610 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: H7R37_RS12325
• Protein ID: WP_000534858.1
• Coordinates: 2557084..2557323 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R37_RS12300	2552346..2552960	+	615	WP_001265627.1	hypothetical protein	-
H7R37_RS12305	2552957..2553985	+	1029	WP_001345551.1	DUF4868 domain-containing protein	-
H7R37_RS12310	2554469..2555782	+	1314	WP_000589005.1	ISNCY family transposase	-
H7R37_RS12315	2556219..2556551	-	333	WP_001301033.1	protein FlxA	-
H7R37_RS12320	2556754..2557059	-	306	WP_001326990.1	hypothetical protein	-
H7R37_RS12325	2557084..2557323	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
H7R37_RS12330	2557323..2557610	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
H7R37_RS12335	2557682..2557837	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
H7R37_RS12340	2558054..2558305	+	252	WP_000980994.1	hypothetical protein	-
H7R37_RS12345	2558372..2558650	+	279	WP_012304870.1	hypothetical protein	-
H7R37_RS12350	2558652..2559701	+	1050	WP_001265199.1	DUF968 domain-containing protein	-
H7R37_RS12355	2559715..2560467	+	753	WP_001047135.1	antitermination protein	-
H7R37_RS12360	2560745..2560834	-	90	WP_120795389.1	hypothetical protein	-
H7R37_RS12365	2560889..2561101	-	213	WP_000087756.1	cold shock-like protein CspF	-
H7R37_RS12370	2561402..2561617	+	216	WP_000066484.1	cold shock-like protein CspB	-
H7R37_RS12375	2562371..2562586	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2530941..2599642	68701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T34923 WP_000323025.1 NZ_AP022207:2557323-2557610 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T34923 NZ_AP022207:2557323-2557610 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT34923 WP_000534858.1 NZ_AP022207:2557084-2557323 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT34923 NZ_AP022207:2557084-2557323 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8