Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 69642..70167 Replicon plasmid pWP7-S17-ESBL-08_2
Accession NZ_AP022185
Organism Klebsiella quasipneumoniae strain WP7-S17-ESBL-08

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag H7R75_RS26250 Protein ID WP_025714517.1
Coordinates 69642..69947 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag H7R75_RS26255 Protein ID WP_001568025.1
Coordinates 69949..70167 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R75_RS26220 65321..65947 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
H7R75_RS26225 65944..66246 + 303 WP_071571079.1 hypothetical protein -
H7R75_RS26230 66718..67512 - 795 WP_004197635.1 site-specific integrase -
H7R75_RS26235 67710..68726 - 1017 WP_017899884.1 hypothetical protein -
H7R75_RS26240 68737..69051 - 315 WP_053389906.1 hypothetical protein -
H7R75_RS26245 69078..69473 - 396 WP_017899885.1 hypothetical protein -
H7R75_RS26250 69642..69947 - 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R75_RS26255 69949..70167 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R75_RS26260 70747..71316 + 570 WP_016162066.1 hypothetical protein -
H7R75_RS26265 71457..72485 + 1029 WP_001568023.1 Abi family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA1 / sul1 / qnrS1 / blaTEM-1B / blaCTX-M-15 - 1..118077 118077


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T34904 WP_025714517.1 NZ_AP022185:c69947-69642 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34904 NZ_AP022185:c69947-69642 [Klebsiella quasipneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT34904 WP_001568025.1 NZ_AP022185:c70167-69949 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34904 NZ_AP022185:c70167-69949 [Klebsiella quasipneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References