Detailed information of TA system TA034902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53068..53804
• Replicon: plasmid pWP7-S17-ESBL-08_1
• Accession: NZ_AP022184
• Organism: Klebsiella quasipneumoniae strain WP7-S17-ESBL-08

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: H7R75_RS24940
• Protein ID: WP_003026803.1
• Coordinates: 53068..53550 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: H7R75_RS24945
• Protein ID: WP_003026799.1
• Coordinates: 53538..53804 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R75_RS24920	48385..49089	-	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
H7R75_RS24925	49249..50595	-	1347	WP_077251107.1	ISNCY family transposase	-
H7R75_RS24930	50825..51457	+	633	WP_001567369.1	hypothetical protein	-
H7R75_RS24935	51464..52867	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
H7R75_RS24940	53068..53550	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
H7R75_RS24945	53538..53804	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
H7R75_RS24950	54249..54479	-	231	WP_004206660.1	hypothetical protein	-
H7R75_RS24955	54493..54696	-	204	WP_004206661.1	hemolysin expression modulator Hha	-
H7R75_RS24960	54757..55248	-	492	WP_004206662.1	hypothetical protein	-
H7R75_RS24965	55382..56593	-	1212	WP_032415728.1	hypothetical protein	-
H7R75_RS24970	56590..56838	-	249	WP_004193994.1	hypothetical protein	-
H7R75_RS24975	57196..57546	+	351	WP_004206664.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
H7R75_RS24980	57598..57960	+	363	WP_004206665.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkJ / mrkF / mrkC / mrkB / mrkA / pla	1..218891	218891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T34902 WP_003026803.1 NZ_AP022184:c53550-53068 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T34902 NZ_AP022184:c53550-53068 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT34902 WP_003026799.1 NZ_AP022184:c53804-53538 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT34902 NZ_AP022184:c53804-53538 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66