Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 4464..4999 Replicon plasmid pWP7-S17-ESBL-04_2
Accession NZ_AP022179
Organism Escherichia coli strain WP7-S17-ESBL-04

Toxin (Protein)


Gene name relE Uniprot ID T2HS21
Locus tag H7R11_RS23385 Protein ID WP_000222775.1
Coordinates 4712..4999 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T2HSA2
Locus tag H7R11_RS23380 Protein ID WP_001139207.1
Coordinates 4464..4715 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R11_RS23345 101..1314 + 1214 WP_162829202.1 IS3 family transposase -
H7R11_RS23350 1387..1644 - 258 Protein_2 IS3 family transposase -
H7R11_RS23355 1668..1814 + 147 WP_072007499.1 type I toxin-antitoxin system Hok family toxin -
H7R11_RS23360 2100..2360 + 261 WP_032291144.1 replication regulatory protein RepA -
H7R11_RS23365 2586..2660 + 75 WP_001365705.1 RepA leader peptide Tap -
H7R11_RS23370 2653..3510 + 858 WP_182913531.1 incFII family plasmid replication initiator RepA -
H7R11_RS23375 4210..4377 + 168 WP_164722579.1 hypothetical protein -
H7R11_RS23380 4464..4715 + 252 WP_001139207.1 plasmid stabilization protein Antitoxin
H7R11_RS23385 4712..4999 + 288 WP_000222775.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R11_RS23390 5009..5554 + 546 WP_170923962.1 hypothetical protein -
H7R11_RS23395 6259..6936 + 678 WP_001339397.1 IS66 family insertion sequence hypothetical protein -
H7R11_RS23400 6936..7283 + 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
H7R11_RS23405 7303..8874 + 1572 WP_000381395.1 IS66 family transposase -
H7R11_RS23410 9165..9512 + 348 WP_087896598.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..94173 94173
- inside IScluster/Tn - - 101..11099 10998


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.17 Da        Isoelectric Point: 10.6261

>T34881 WP_000222775.1 NZ_AP022179:4712-4999 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Download         Length: 288 bp

>T34881 NZ_AP022179:4712-4999 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGTGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9216.45 Da        Isoelectric Point: 4.0927

>AT34881 WP_001139207.1 NZ_AP022179:4464-4715 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT34881 NZ_AP022179:4464-4715 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGTTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB T2HS21

Antitoxin

Source ID Structure
AlphaFold DB A0A754EQ65

References