Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 37552..38153 Replicon plasmid pWP7-S17-ESBL-04_1
Accession NZ_AP022178
Organism Escherichia coli strain WP7-S17-ESBL-04

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag H7R11_RS22890 Protein ID WP_001216034.1
Coordinates 37552..37932 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag H7R11_RS22895 Protein ID WP_001190712.1
Coordinates 37932..38153 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R11_RS22855 32603..33535 - 933 WP_000081352.1 homocysteine S-methyltransferase -
H7R11_RS22860 33522..34925 - 1404 WP_001373486.1 S-methylmethionine permease -
H7R11_RS22865 35133..36149 + 1017 Protein_35 IS5 family transposase -
H7R11_RS22870 36377..36694 + 318 WP_001513659.1 hypothetical protein -
H7R11_RS22875 36644..37057 - 414 Protein_37 DDE-type integrase/transposase/recombinase -
H7R11_RS22880 36981..37340 - 360 WP_072617413.1 pdcB -
H7R11_RS22885 37368..37547 - 180 WP_001513661.1 hypothetical protein -
H7R11_RS22890 37552..37932 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
H7R11_RS22895 37932..38153 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H7R11_RS22900 38416..39113 + 698 Protein_42 IS1-like element IS1A family transposase -
H7R11_RS22905 39300..39557 - 258 WP_072617411.1 biofilm development YmgB/AriR family protein -
H7R11_RS22910 39897..40001 - 105 Protein_44 IS3 family transposase -
H7R11_RS22915 40320..41017 - 698 Protein_45 IS1 family transposase -
H7R11_RS22920 41391..42005 + 615 WP_032214276.1 acid-sensing system DNA-binding response regulator EvgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrS1 / blaCTX-M-15 / blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..123262 123262
- inside IScluster/Tn - - 30569..40715 10146


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T34880 WP_001216034.1 NZ_AP022178:c37932-37552 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T34880 NZ_AP022178:c37932-37552 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT34880 WP_001190712.1 NZ_AP022178:c38153-37932 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT34880 NZ_AP022178:c38153-37932 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References