Detailed information of TA system TA034876

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3642648..3643342
• Replicon: chromosome
• Accession: NZ_AP022177
• Organism: Escherichia coli strain WP7-S17-ESBL-04

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A2A3UTQ9
• Locus tag: H7R11_RS17560
• Protein ID: WP_001094399.1
• Coordinates: 3642648..3643016 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: H7R11_RS17565
• Protein ID: WP_077249034.1
• Coordinates: 3643037..3643342 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R11_RS17540	3637821..3638777	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
H7R11_RS17545	3638774..3640972	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
H7R11_RS17550	3640982..3641938	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
H7R11_RS17555	3641989..3642327	+	339	Protein_3434	LysR family transcriptional regulator	-
H7R11_RS17560	3642648..3643016	-	369	WP_001094399.1	TA system toxin CbtA family protein	Toxin
H7R11_RS17565	3643037..3643342	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H7R11_RS17570	3643446..3644090	-	645	WP_000086755.1	hypothetical protein	-
H7R11_RS17575	3644109..3644330	-	222	WP_000692315.1	DUF987 domain-containing protein	-
H7R11_RS17580	3644393..3644869	-	477	WP_001186773.1	RadC family protein	-
H7R11_RS17585	3644885..3645370	-	486	WP_001737493.1	antirestriction protein	-
H7R11_RS17590	3645405..3646976	-	1572	WP_000381395.1	IS66 family transposase	-
H7R11_RS17595	3646996..3647343	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
H7R11_RS17600	3647343..3648020	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13568.73 Da
• Isoelectric Point: 7.7465

>T34876 WP_001094399.1 NZ_AP022177:c3643016-3642648 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T34876 NZ_AP022177:c3643016-3642648 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT34876 WP_077249034.1 NZ_AP022177:c3643342-3643037 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT34876 NZ_AP022177:c3643342-3643037 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A2A3UTQ9