Detailed information of TA system TA034873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2262579..2263105
• Replicon: chromosome
• Accession: NZ_AP022177
• Organism: Escherichia coli strain WP7-S17-ESBL-04

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: H7R11_RS10795
• Protein ID: WP_000323025.1
• Coordinates: 2262818..2263105 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: H7R11_RS10790
• Protein ID: WP_000534858.1
• Coordinates: 2262579..2262818 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R11_RS10770	2257941..2258297	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
H7R11_RS23950	2258908..2259195	+	288	Protein_2106	hypothetical protein	-
H7R11_RS10775	2259964..2261277	+	1314	WP_000589005.1	ISNCY family transposase	-
H7R11_RS10780	2261714..2262046	-	333	WP_001301033.1	protein FlxA	-
H7R11_RS10785	2262249..2262554	-	306	WP_001326990.1	hypothetical protein	-
H7R11_RS10790	2262579..2262818	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
H7R11_RS10795	2262818..2263105	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
H7R11_RS10800	2263177..2263332	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
H7R11_RS10805	2263549..2263800	+	252	WP_000980994.1	hypothetical protein	-
H7R11_RS10810	2263867..2264145	+	279	WP_012304870.1	hypothetical protein	-
H7R11_RS10815	2264147..2265196	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
H7R11_RS10820	2265210..2265962	+	753	WP_001047135.1	antitermination protein	-
H7R11_RS10825	2266240..2266329	-	90	WP_120795389.1	hypothetical protein	-
H7R11_RS10830	2266384..2266596	-	213	WP_000087756.1	cold shock-like protein CspF	-
H7R11_RS10835	2266897..2267112	+	216	WP_000066484.1	cold shock-like protein CspB	-
H7R11_RS10840	2267866..2268081	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2237705..2320008	82303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T34873 WP_000323025.1 NZ_AP022177:2262818-2263105 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T34873 NZ_AP022177:2262818-2263105 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT34873 WP_000534858.1 NZ_AP022177:2262579-2262818 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT34873 NZ_AP022177:2262579-2262818 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8