Detailed information of TA system TA034854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 52436..52995
• Replicon: plasmid pWP7-S17-ESBL-01_1
• Accession: NZ_AP022174
• Organism: Escherichia coli strain WP7-S17-ESBL-01

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: H7R26_RS25225
• Protein ID: WP_000421257.1
• Coordinates: 52720..52995 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: H7R26_RS25220
• Protein ID: WP_001178087.1
• Coordinates: 52436..52720 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R26_RS25195	47780..48022	-	243	WP_021515452.1	PilI type IV pilus biogenesis protein	-
H7R26_RS25200	48103..48246	-	144	WP_000176500.1	hypothetical protein	-
H7R26_RS25205	48513..49175	-	663	WP_005082527.1	hypothetical protein	-
H7R26_RS25210	49315..49956	-	642	WP_021515453.1	hypothetical protein	-
H7R26_RS25215	50659..51531	+	873	WP_000729438.1	incFII family plasmid replication initiator RepA	-
H7R26_RS25220	52436..52720	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
H7R26_RS25225	52720..52995	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R26_RS25230	53160..53441	+	282	WP_001105066.1	hypothetical protein	-
H7R26_RS25235	53632..53898	-	267	WP_000627831.1	hypothetical protein	-
H7R26_RS25240	54036..54638	-	603	WP_000521606.1	hypothetical protein	-
H7R26_RS25245	54814..55866	-	1053	WP_000655519.1	hypothetical protein	-
H7R26_RS25250	56079..56669	-	591	WP_001401589.1	hypothetical protein	-
H7R26_RS25255	56669..56923	-	255	WP_000343106.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..94776	94776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T34854 WP_000421257.1 NZ_AP022174:52720-52995 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T34854 NZ_AP022174:52720-52995 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT34854 WP_001178087.1 NZ_AP022174:52436-52720 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT34854 NZ_AP022174:52436-52720 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCTCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: U9Y625