Detailed information of TA system TA034841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2729928..2730566
• Replicon: chromosome
• Accession: NZ_AP022173
• Organism: Escherichia coli strain WP7-S17-ESBL-01

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: H7R26_RS13220
• Protein ID: WP_000813797.1
• Coordinates: 2730390..2730566 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H7R26_RS13215
• Protein ID: WP_076611057.1
• Coordinates: 2729928..2730344 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R26_RS13195	2725079..2726020	-	942	WP_001251335.1	ABC transporter permease	-
H7R26_RS13200	2726021..2727034	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
H7R26_RS13205	2727052..2728198	-	1147	Protein_2593	ABC transporter substrate-binding protein	-
H7R26_RS13210	2728443..2729849	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
H7R26_RS13215	2729928..2730344	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H7R26_RS13220	2730390..2730566	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H7R26_RS13225	2730788..2731018	+	231	WP_000494244.1	YncJ family protein	-
H7R26_RS13230	2731110..2733071	-	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H7R26_RS13235	2733144..2733680	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
H7R26_RS13240	2733733..2734944	+	1212	WP_001323162.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T34841 WP_000813797.1 NZ_AP022173:c2730566-2730390 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T34841 NZ_AP022173:c2730566-2730390 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT34841 WP_076611057.1 NZ_AP022173:c2730344-2729928 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT34841 NZ_AP022173:c2730344-2729928 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A829J715