Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 157754..158279 Replicon plasmid pWP5-S18-ESBL-09_1
Accession NZ_AP022172
Organism Escherichia coli strain WP5-S18-ESBL-09

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H7R27_RS25245 Protein ID WP_001159871.1
Coordinates 157754..158059 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H7R27_RS25250 Protein ID WP_000813634.1
Coordinates 158061..158279 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R27_RS25210 153181..153978 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
H7R27_RS25215 154040..154291 + 252 Protein_197 DUF4113 domain-containing protein -
H7R27_RS25220 154296..154688 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
H7R27_RS25225 154693..155664 - 972 WP_001103694.1 hypothetical protein -
H7R27_RS25230 155893..156537 + 645 WP_000633911.1 AAA family ATPase -
H7R27_RS25235 156531..156806 + 276 WP_000239529.1 hypothetical protein -
H7R27_RS25240 156944..157753 - 810 WP_000016979.1 site-specific integrase -
H7R27_RS25245 157754..158059 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R27_RS25250 158061..158279 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R27_RS25255 160601..161242 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B senB 1..187669 187669


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T34821 WP_001159871.1 NZ_AP022172:c158059-157754 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34821 NZ_AP022172:c158059-157754 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT34821 WP_000813634.1 NZ_AP022172:c158279-158061 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34821 NZ_AP022172:c158279-158061 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References