34821

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 157754..158279 Replicon plasmid pWP5-S18-ESBL-09_1
Accession NZ_AP022172
Organism Escherichia coli strain WP5-S18-ESBL-09

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H7R27_RS25245 Protein ID WP_001159871.1
Coordinates 157754..158059 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H7R27_RS25250 Protein ID WP_000813634.1
Coordinates 158061..158279 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R27_RS25210 153181..153978 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
H7R27_RS25215 154040..154291 + 252 Protein_197 DUF4113 domain-containing protein -
H7R27_RS25220 154296..154688 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
H7R27_RS25225 154693..155664 - 972 WP_001103694.1 hypothetical protein -
H7R27_RS25230 155893..156537 + 645 WP_000633911.1 AAA family ATPase -
H7R27_RS25235 156531..156806 + 276 WP_000239529.1 hypothetical protein -
H7R27_RS25240 156944..157753 - 810 WP_000016979.1 site-specific integrase -
H7R27_RS25245 157754..158059 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R27_RS25250 158061..158279 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R27_RS25255 160601..161242 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B senB 1..187669 187669


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T34821 WP_001159871.1 NZ_AP022172:c158059-157754 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34821 NZ_AP022172:c158059-157754 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT34821 WP_000813634.1 NZ_AP022172:c158279-158061 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34821 NZ_AP022172:c158279-158061 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References