Detailed information of TA system TA034811

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4514919..4515754
• Replicon: chromosome
• Accession: NZ_AP022171
• Organism: Escherichia coli strain WP5-S18-ESBL-09

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q8VR61
• Locus tag: H7R27_RS21750
• Protein ID: WP_000854747.1
• Coordinates: 4515377..4515754 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A6A0PK89
• Locus tag: H7R27_RS21745
• Protein ID: WP_024186867.1
• Coordinates: 4514919..4515287 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R27_RS21710	4510831..4511511	+	681	WP_001282919.1	WYL domain-containing protein	-
H7R27_RS21715	4511659..4512336	+	678	WP_001097301.1	hypothetical protein	-
H7R27_RS21720	4512342..4512575	+	234	WP_001278280.1	DUF905 family protein	-
H7R27_RS21725	4512665..4513483	+	819	WP_001175142.1	DUF945 domain-containing protein	-
H7R27_RS21730	4513574..4514059	+	486	WP_001588934.1	antirestriction protein	-
H7R27_RS21735	4514074..4514550	+	477	WP_001588933.1	RadC family protein	-
H7R27_RS21740	4514619..4514840	+	222	WP_000692312.1	DUF987 domain-containing protein	-
H7R27_RS21745	4514919..4515287	+	369	WP_024186867.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
H7R27_RS21750	4515377..4515754	+	378	WP_000854747.1	TA system toxin CbtA family protein	Toxin
H7R27_RS21755	4515751..4516239	+	489	WP_000761667.1	hypothetical protein	-
H7R27_RS21760	4516251..4516448	+	198	WP_000839291.1	DUF957 domain-containing protein	-
H7R27_RS21765	4516533..4517387	+	855	WP_001588930.1	DUF4942 domain-containing protein	-
H7R27_RS21770	4518124..4519662	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4502235..4543158	40923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14095.03 Da
• Isoelectric Point: 6.8524

>T34811 WP_000854747.1 NZ_AP022171:4515377-4515754 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNDITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T34811 NZ_AP022171:4515377-4515754 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACCCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATGACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13555.35 Da
• Isoelectric Point: 6.4745

>AT34811 WP_024186867.1 NZ_AP022171:4514919-4515287 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGCVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT34811 NZ_AP022171:4514919-4515287 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGATACCCTCGGCAGCTG
TGGTTGCGTTTATCTGGCTGTTTATCCGACACCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0H2VFZ1

Antitoxin

• AlphaFold DB: A0A6A0PK89