Detailed information of TA system TA034810

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4203119..4203953
• Replicon: chromosome
• Accession: NZ_AP022171
• Organism: Escherichia coli strain WP5-S18-ESBL-09

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: H7R27_RS20265
• Protein ID: WP_000854770.1
• Coordinates: 4203119..4203496 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M1N885
• Locus tag: H7R27_RS20270
• Protein ID: WP_001280950.1
• Coordinates: 4203585..4203953 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R27_RS20240	4199230..4200870	-	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-
H7R27_RS20245	4201643..4201819	-	177	Protein_3972	helix-turn-helix domain-containing protein	-
H7R27_RS20250	4202183..4202341	-	159	WP_001467148.1	hypothetical protein	-
H7R27_RS20255	4202441..4202617	-	177	WP_000839286.1	DUF957 domain-containing protein	-
H7R27_RS20260	4202634..4203122	-	489	WP_000761690.1	hypothetical protein	-
H7R27_RS20265	4203119..4203496	-	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
H7R27_RS20270	4203585..4203953	-	369	WP_001280950.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H7R27_RS20275	4204116..4204337	-	222	WP_001220314.1	DUF987 domain-containing protein	-
H7R27_RS20280	4204400..4204876	-	477	WP_001186779.1	RadC family protein	-
H7R27_RS20285	4204892..4205356	-	465	WP_000855061.1	antirestriction protein	-
H7R27_RS20290	4205698..4206516	-	819	WP_001234712.1	DUF945 domain-containing protein	-
H7R27_RS20295	4206671..4206829	-	159	WP_001323397.1	DUF905 family protein	-
H7R27_RS20300	4206900..4208801	-	1902	Protein_3983	autotransporter adhesin Ag43	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T34810 WP_000854770.1 NZ_AP022171:c4203496-4203119 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T34810 NZ_AP022171:c4203496-4203119 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.56 Da
• Isoelectric Point: 7.0268

>AT34810 WP_001280950.1 NZ_AP022171:c4203953-4203585 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT34810 NZ_AP022171:c4203953-4203585 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9

Antitoxin

• AlphaFold DB: A0A1M1N885