Detailed information of TA system TA034809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4171750..4172570
• Replicon: chromosome
• Accession: NZ_AP022171
• Organism: Escherichia coli strain WP5-S18-ESBL-09

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: H7R27_RS20115
• Protein ID: WP_001054379.1
• Coordinates: 4171750..4172007 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: H7R27_RS20120
• Protein ID: WP_000123957.1
• Coordinates: 4172019..4172570 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R27_RS20085	4167230..4167733	-	504	Protein_3939	DNA helicase	-
H7R27_RS20090	4167819..4168244	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
H7R27_RS20095	4168241..4168591	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
H7R27_RS20100	4168622..4170235	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
H7R27_RS20105	4170265..4170996	-	732	Protein_3943	DNA helicase	-
H7R27_RS20110	4171074..4171373	+	300	WP_001332034.1	GNAT family N-acetyltransferase	-
H7R27_RS20115	4171750..4172007	+	258	WP_001054379.1	YjhX family toxin	Toxin
H7R27_RS20120	4172019..4172570	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
H7R27_RS20125	4172622..4173368	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
H7R27_RS20130	4173496..4173756	+	261	WP_000077645.1	hypothetical protein	-
H7R27_RS25410	4173794..4173910	+	117	Protein_3949	VOC family protein	-
H7R27_RS20135	4174155..4175276	+	1122	WP_000010833.1	M42 family metallopeptidase	-
H7R27_RS20140	4175273..4175551	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
H7R27_RS20145	4175563..4176876	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T34809 WP_001054379.1 NZ_AP022171:4171750-4172007 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T34809 NZ_AP022171:4171750-4172007 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT34809 WP_000123957.1 NZ_AP022171:4172019-4172570 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT34809 NZ_AP022171:4172019-4172570 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030