Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4132263..4132839 Replicon chromosome
Accession NZ_AP022171
Organism Escherichia coli strain WP5-S18-ESBL-09

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag H7R27_RS19910 Protein ID WP_001297643.1
Coordinates 4132552..4132839 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag H7R27_RS19905 Protein ID WP_000063156.1
Coordinates 4132263..4132565 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R27_RS19890 4128859..4131009 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
H7R27_RS19895 4131059..4131262 + 204 WP_000467859.1 YbdD/YjiX family protein -
H7R27_RS19900 4131273..4132229 + 957 WP_001297640.1 GTPase -
H7R27_RS19905 4132263..4132565 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
H7R27_RS19910 4132552..4132839 - 288 WP_001297643.1 BrnT family toxin Toxin
H7R27_RS19915 4133038..4133735 - 698 Protein_3905 IS1 family transposase -
H7R27_RS19920 4133792..4133887 + 96 Protein_3906 dienelactone hydrolase family protein -
H7R27_RS19925 4134033..4135265 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
H7R27_RS19930 4135306..4136586 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T34808 WP_001297643.1 NZ_AP022171:c4132839-4132552 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T34808 NZ_AP022171:c4132839-4132552 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT34808 WP_000063156.1 NZ_AP022171:c4132565-4132263 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT34808 NZ_AP022171:c4132565-4132263 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References