Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 94353..94954 Replicon plasmid pWP5-S18-ESBL-08_2
Accession NZ_AP022163
Organism Escherichia coli strain WP5-S18-ESBL-08

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag H7R36_RS26195 Protein ID WP_001216045.1
Coordinates 94353..94733 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag H7R36_RS26200 Protein ID WP_001190712.1
Coordinates 94733..94954 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R36_RS26170 89794..91278 - 1485 WP_000124150.1 terminase -
H7R36_RS26175 91278..92471 - 1194 WP_186225988.1 terminase -
H7R36_RS26180 92557..93009 - 453 WP_001326849.1 late promoter-activating protein -
H7R36_RS26185 93098..94141 - 1044 WP_023356283.1 DUF968 domain-containing protein -
H7R36_RS26190 94169..94348 - 180 WP_001339207.1 hypothetical protein -
H7R36_RS26195 94353..94733 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
H7R36_RS26200 94733..94954 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H7R36_RS26205 95027..95416 - 390 WP_000506730.1 DNA repair protein -
H7R36_RS26210 95591..96175 + 585 WP_186225985.1 DNA-binding protein -
H7R36_RS26215 96176..96538 + 363 WP_053273046.1 hypothetical protein -
H7R36_RS26220 96550..96789 - 240 WP_001423760.1 DNA polymerase III subunit theta -
H7R36_RS26225 97579..97794 - 216 WP_000466686.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..97833 97833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T34784 WP_001216045.1 NZ_AP022163:c94733-94353 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T34784 NZ_AP022163:c94733-94353 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCTGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT34784 WP_001190712.1 NZ_AP022163:c94954-94733 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT34784 NZ_AP022163:c94954-94733 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References